PROJECT SUMMARY The Neuroproteomics Core of The Scripps Research Institute Alcohol Research Center (TSRI-ARC) will provide mass spectrometry-based bioanalytical collaborations to meet the specific needs of the TSRI-ARC and Center- at-Large. Given the breadth of work to be conducted in this translational research center, it is understandable that not all personnel will have expertise in specialized areas of analytical chemistry. The first goal of the Neuroproteomics Core is to provide access to proteomic methods, such as protein expression analysis, phosphoproteomics, protein interactions, and biorthogonal chemistry, for the discovery of molecular mechanisms in animal models of alcohol drinking and dependence to TSRI-ARC investigators. Personalized consultation with investigators on issues related to experimental design, sample collection, sample handling, and data interpretation will be provided. The second goal of the Core is to advance methods to perform single-neuron proteomic measurements in combination with electrophysiology measurements to study alcohol use disorder (AUD). In all these experiments, Core personnel will work closely with TSRI-ARC investigators to solve technical problems and assess and analyze the data.