The objective of the Technology Core is to provide state-of-the-art, innovative -OMICs technologies for the targeted and global characterization of Project samples in a quantitative, reproducible, and efficient manner. The core brings together cutting-edge tools and the expertise of leading systems biologists in three critical areas: (1) Proteomics led by Dr. Nevan Krogan at the J. David Gladstone Institutes; (2) Transcriptomics and Epigenetics led by Drs. Chris Benner and Ivan Marazzi at the University of California, San Diego and the Icahn School of Medicine at Mount Sinai; and (3) Viral Reverse Genetics led by Dr. Luis Martinez-Sobrido at the Texas Biomedical Research Institute. The Technology Core will be responsible for processing and analyzing both in vivo samples from infected patients and mice (Project 1) as well as ex vivo infected samples in primary and immortalized cells (Projects 1 and 2). The generated data will be processed, analyzed and stored in collaboration with the Data Management and Bioinformatics Core. Data integration will be performed by the Modeling Core to discover key drivers of coronavirus infection, transmission, host response, and host tropism as potential nodes for therapeutic intervention. Further integration of -OMICs data with longitudinal clinical data derived from patient cohorts will identify biomarkers of disease severity. The Technology Core will provide recombinant viruses (including reporter, deletion, and point mutation) and -OMICs services for essential functional and mechanistic follow-up studies probing the molecular mechanism of the critical drivers identified in each respective Project. This iterative cycle from networks-to-mechanism is driven by our effective collaborative efforts that unites multiple labs to optimally leverage best-in-class -OMICs approaches that rely on high-level specialized equipment and domain-specific expertise. The Technology Core has the unique challenge of processing coordinated samples and generating and integrating systems data spanning chromatin modifications and architecture, gene expression, protein abundance, post-translational modifications, and protein-protein interactions. In the previous iteration (the FluOMICs consortium), Drs. Benner and Krogan have successfully tackled these challenges and derived standardized protocols for sample generation, processing, and analysis across different cores and institutes. More recently, Drs. Marazzi and Martinez-Sobrido, who have already established relationships with FluOMICs team members, joined new collaborative efforts to study coronaviruses. Formalizing this relationship as a single core under the leadership of Dr. Krogan, an internationally recognized expert in the design and application of systems biology approaches to interrogate host-pathogen interactions, the Technology Core is ideally positioned to effectively coordinate Technology Core labs to implement previously developed pipelines under FluOMICS 1.0 and 2.0 and d...