# Transgenic Core

> **NIH NIH P01** · FOOD ALLERGY SCIENCE INITIATIVE, INC. · 2024 · $345,800

## Abstract

ABSTRACT
The P01 Transgenic Core (PI – Bradford Lowell, BIDMC) will work with the Food Allergy P01 investigators to
create genetically engineered mice as outlined in their three projects. A complete suite of services are
available, including the ability to engineer transgenic, gene knockout and gene knockin mice. Two common
forms of gene knockin mice include recombinase driver mice and mice bearing lox-modified alleles. For
recombinase-driver mice, “marker” genes known to be neuron subtype- or cell subtype-specific, are
engineered to drive expression of DNA recombinases (e.g. Cre recombinase). Once generated, such mice are
used with recombinase-dependent tools to monitor, map and manipulate the unique subtypes of neurons and
cells. In the case of mice bearing lox-modified alleles, functionally important genes are engineered to be
flanked by lox sites. This makes it possible to selectively delete that gene from any cre-expressing neuron or
cell. Of note, the CRISPR-based genetic engineering strategy being employed can be done in any mouse
strain, including BALB/c which is an important strain for food allergy research. Additional services available
include mouse ES cell-based gene targeting, cryopreservation of mutant mice in the form of frozen embryos or
frozen sperm and resurrection of mutant mice from frozen embryos or frozen sperm. The Transgenic Core PI,
Bradford Lowell, has many years of experience in the generation and utilization of genetically engineered mice
to study neuronal function, including that of vagal motor neurons that innervate the gut. The BIDMC
Transgenic Facility manager, Joel Lawitts, has many years of experience in culturing and manipulating
preimplantation mouse embryos, and in running the BIDMC Transgenic Facility. Together, Lowell, Lawitts and
the BIDMC Transgenic Facility have provided extensive mouse genetic engineering services for many years to
numerous investigators from many different institutions. Indeed, from 2016 to present, 188 services (mostly
CRISPR projects) have been provided to 39 different investigators from 12 different institutions. Hence, this
P01 Transgenic Core has vast experience and can meet all the genetic engineering needs of the Food Allergy
P01 investigators.

## Key facts

- **NIH application ID:** 10762660
- **Project number:** 1P01AI179273-01
- **Recipient organization:** FOOD ALLERGY SCIENCE INITIATIVE, INC.
- **Principal Investigator:** BRADFORD B LOWELL
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $345,800
- **Award type:** 1
- **Project period:** 2024-05-14 → 2029-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10762660

## Citation

> US National Institutes of Health, RePORTER application 10762660, Transgenic Core (1P01AI179273-01). Retrieved via AI Analytics 2026-05-26 from https://api.ai-analytics.org/grant/nih/10762660. Licensed CC0.

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