PROJECT SUMMARY/ABSTRACT The Preclinical Imaging Laboratory Shared Resource (PCI) mission is to foster cutting-edge research by providing highly specialized expertise and service in noninvasive rodent imaging to Perlmutter Cancer Center (PCC) members in a collaborative environment. Under the expert direction of Youssef Wadghiri, PhD, PCI provides rodent imaging modalities equivalent to those available in clinical settings. Wadghiri has 32 years of experience in imaging, 11 years as shared resource director, and an international reputation in developing molecular imaging strategies, image-guided interventions, instruments, and molecular probes. PCI is further staffed by an Assistant Director and 3 highly trained scientists. PCI has continued to update its instrument fleet with 6 new scanners, resulting in a total of 18 instruments across 4 buildings throughout the NYU Grossman School of Medicine campus, while expanding its imaging capabilities from 5 to 7 modalities. The wide variety of available imaging modalities (MRI, PET, SPECT, CT, Bioluminescence/Fluorescence imaging, high frequency ultrasound, and dual energy X-ray absorptiometry) facilitate cost-effective, noninvasive screening and longitudinal examination of all rodent organs in cancer models at the anatomical, metabolic, and molecular levels. PCI is critical to the research of three PCC Research Programs, CGD, CCB, and TIM; during the 2018-2022 funding cycle, 45 peer-reviewed funded grants and 76 publications used PCI resources and expertise. We regularly assess PCC member needs and satisfaction via surveys and an internal advisory board, and ensure best practices, quality control, instrument maintenance, and data provenance for scientific rigor and transparency. The Specific Aims of PCI are: 1) to provide multiple small animal imaging instruments and technologies to PCC members, including MRI, PET, SPECT, CT, high frequency ultrasound, DEXA, and bioluminescence and fluorescence imaging, 2) to provide PCC researchers with specific expertise, i.e., assist with experimental design, direct instrumentation technical support, and expert data analysis and interpretation, and to facilitate data sharing and management through state-of-the-art software and high-performance, secure servers, 3) to implement novel technologies and methodologies for cutting-edge imaging support including interventional imaging approaches, and 4) to foster an educational and training environment to the PCC research community on learning how imaging technology can help advance their cancer research.