# Advanced Biophotonics Core (BPHO)

> **NIH NIH P30** · SALK INSTITUTE FOR BIOLOGICAL STUDIES · 2024 · $222,266

## Abstract

PROJECT SUMMARY – ADVANCED BIOPHOTONICS
The Advanced Biophotonics Shared Resource (BPHO) provides imaging and analysis instrumentation coupled
with technical and collaborative support staff for advanced light and electron microscopy of biological systems.
In addition to the Director, the BPHO is staffed by three full-time imaging specialists who have expertise in
electron and light microscopy, as well as data analysis. Salk Cancer Center (SCC) members use the facility for
high-throughput imaging assays, high-resolution imaging of live cell and tissue dynamics, super-resolution
microscopy, large 3D volume imaging of tissues, electron microscopy analysis of subcellular morphology and
protein distribution, and automated computational image processing, visualization, and analysis. The BPHO is
also actively pursuing and developing new cutting-edge imaging and analysis methodologies to better serve the
needs of SCC researchers, such as cryo-correlative light and electron microscopy, light-sheet imaging of cleared
and expanded tissues, and machine-learning based processing, segmentation, and analysis of light and electron
microscope images.
BPHO is committed to providing SCC members:
 1) Access to light and electron microscopes, specialized sample preparation reagents and technologies,
 and computational hardware and software for analysis and visualization
 2) Free one-on-one training on all microscopes, as well as image processing and analysis software
 3) Consulting and collaborative support for experimental design and implementation of imaging and analysis
experiments
 4) Sample preparation for electron microscopy, tissue clearing, and expansion microscopy
 5) Workshops and demos with advanced microscopy and software technologies
 6) Weekly open-door imaging boot camps on advanced imaging and image processing techniques
 7) A monthly Biophotonics scientific seminar series followed by town-hall discussion with BPHO staff and
users
Utilization of the imaging technologies and expertise in this Shared Resource is high. During the current funding
period, eighty-one percent (81%) of the SCC members have benefited from utilization of the facility. This
accounts for approximately 61% of total BPHO utilization.

## Key facts

- **NIH application ID:** 10769670
- **Project number:** 2P30CA014195-51
- **Recipient organization:** SALK INSTITUTE FOR BIOLOGICAL STUDIES
- **Principal Investigator:** GERALD SHADEL
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $222,266
- **Award type:** 2
- **Project period:** 1996-12-31 → 2029-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10769670

## Citation

> US National Institutes of Health, RePORTER application 10769670, Advanced Biophotonics Core (BPHO) (2P30CA014195-51). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10769670. Licensed CC0.

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