# Flow Cytometry Core (FCC)

> **NIH NIH P30** · SALK INSTITUTE FOR BIOLOGICAL STUDIES · 2024 · $192,461

## Abstract

PROJECT SUMMARY – FLOW CYTOMETRY
The Flow Cytometry Shared Resource (FCC) provides Salk investigators access to state-of-the-art flow
cytometry instrumentation, high quality services (ensuring that all samples are manipulated and analyzed
appropriately), and expert consultation concerning the design and implementation of flow-based experiments.
Flow cytometry is a powerful single-cell technique that is widely employed at the Salk Institute with 81% (25/31)
of Salk Cancer Center (SCC) members utilizing these services. This accounts for 83% of FCC total usage based
on recharges. Cell sorting is often used as an upstream mechanism for single-cell sequencing or cell implantation
studies, critical for cancer research and understanding complex tumor microenvironments and heterogeneity.
Multi-color analysis can be applied to a diverse array of disease models involving multiple cell types. In recent
years, advancements in instrumentation and fluorescence technologies have allowed complex experiments
involving more than 20 different labels to be utilized. The FCC offers two categories of flow cytometry
instruments: 1) instruments capable of sample purification (commonly referred to as “cell-sorters” or “sorters”),
and 2) analytical instruments. The staff fosters a collaborative environment, striving to provide an easily
accessible and comprehensive resource to the SCC, and dedicates effort to identify emerging flow technologies
or applications that will be useful for FCC users. The FCC strives to facilitate SCC research by: 1) Offering
instrumentation – FCC maintains a suite of instruments designed to meet the broad range of current and
anticipated needs of SCC scientists (based on research developments and trends) including critical cell-sorting
services. FCC staff provides high-quality, live cell-sorting services that are tailored to meet the varying needs of
SCC scientists. 2) Providing expertise – The highly experienced FCC staff is readily accessible to SCC
investigators for the collaborative development of assays and/or protocols. 3) Providing education, training, and
support – SCC researchers at all levels of expertise can receive training in all aspects of flow cytometry, from
experimental design to data analysis. And 4) Identifying and evaluating new technologies – The FCC seeks to
continuously ensure that state of-the- art flow capabilities are offered, thereby maximizing user benefit.

## Key facts

- **NIH application ID:** 10769671
- **Project number:** 2P30CA014195-51
- **Recipient organization:** SALK INSTITUTE FOR BIOLOGICAL STUDIES
- **Principal Investigator:** Ye Zheng
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $192,461
- **Award type:** 2
- **Project period:** 1996-12-31 → 2029-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10769671

## Citation

> US National Institutes of Health, RePORTER application 10769671, Flow Cytometry Core (FCC) (2P30CA014195-51). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10769671. Licensed CC0.

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