# Metabolomic characterization of normal aging and Alzheimer's disease under anesthesia- surgery stimuli

> **NIH NIH R01** · MASSACHUSETTS GENERAL HOSPITAL · 2024 · $786,689

## Abstract

We propose to quantify aging- and AD-associated brain and blood metabolic changes through ex vivo and in
vivo multimodality MR measurements in mouse models after an AD-promoting stimuli, anesthesia-surgery,
with-or-without preventive, protective treatments. This approach recognizes that AD onset, pathogenesis and
progression must produce alterations in the overall metabolic status, or metabolomics, of brain and other
organs in AD patients, as compared with normal homeostasis in healthy individuals, and our understanding of
the urgent need to develop non-invasive AD diagnosis tools. In the three proposed Aims, we will (1)
Characterize age-associated metabolomic changes ex vivo using tissues from different brain regions and blood
serum obtained from wild-type and AD mice after administration of anesthesia-surgery stimuli; (2) Develop
multimodality in vivo MR protocols for non-invasive evaluations of age- and AD-associated brain metabolic
changes induced by anesthesia-surgery stimuli; and (3) Investigate the effect of an AD protective intervention
on metabolomic changes after administration of anesthesia-surgery stimuli. The success of this project will
lead us to establish translational protocols that can guide and interpret multimodality in vivo MR imaging results
by obtaining accurate ex vivo MRS, EM, and immunochemistry measurements in the proposed animal models,
and translating these findings into clinical protocols for human AD non-invasive diagnosis.

## Key facts

- **NIH application ID:** 10770362
- **Project number:** 5R01AG070257-04
- **Recipient organization:** MASSACHUSETTS GENERAL HOSPITAL
- **Principal Investigator:** Bruce G Jenkins
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $786,689
- **Award type:** 5
- **Project period:** 2021-02-15 → 2026-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10770362

## Citation

> US National Institutes of Health, RePORTER application 10770362, Metabolomic characterization of normal aging and Alzheimer's disease under anesthesia- surgery stimuli (5R01AG070257-04). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10770362. Licensed CC0.

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