# Scientific Core One

> **NIH NIH U19** · FRED HUTCHINSON CANCER CENTER · 2024 · $126,171

## Abstract

Abstract
Scientific Core One has the responsibility of creating, validating, and providing R&D-grade HIV-Env
immunogen-expressing self-amplifying RNAs (saRNAs) complexed with the nanostructured lipid carrier (NLC)
delivery vehicle to project leads at Fred Hutchinson Cancer Research Center for in vivo testing as described in
Scientific Project One. AAHI’s saRNA/NLC vaccine platform has previously demonstrated robust
immunogenicity against many antigen targets, including HIV, and unparalleled stability. This technology will
now be adapted to deliver the HIV immunogens of our partners at the Fred Hutchinson Cancer Research
Center. Scientific Core One activities include design and cloning of the saRNA template DNA plasmids, saRNA
production using well-characterized and validated in vitro transcription and purification processes, and
manufacturing of the NLC delivery formulation. Upon successful saRNA and NLC manufacture, Scientific Core
One will complex of saRNA/NLC vaccine and test these vaccines in vitro to verify successful saRNA replication
and antigen production. Scientific Core One will also provide transfected cell materials to Scientific Core Two
for immunogen antigenicity testing. Finally, the stability of these R&D-grade saRNA, NLC, and complexed
saRNA/NLC materials will be verified using standard stability testing techniques to gather necessary data to
enable GMP manufacturing by Scientific Project Two, successful filing of an IND and completion of a Phase I
clinical trial.

## Key facts

- **NIH application ID:** 10771238
- **Project number:** 5U19AI174242-02
- **Recipient organization:** FRED HUTCHINSON CANCER CENTER
- **Principal Investigator:** Corey Casper
- **Activity code:** U19 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $126,171
- **Award type:** 5
- **Project period:** 2023-01-30 → 2027-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10771238

## Citation

> US National Institutes of Health, RePORTER application 10771238, Scientific Core One (5U19AI174242-02). Retrieved via AI Analytics 2026-06-08 from https://api.ai-analytics.org/grant/nih/10771238. Licensed CC0.

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