# Scientific Core Two

> **NIH NIH U19** · FRED HUTCHINSON CANCER CENTER · 2024 · $493,694

## Abstract

PROJECT SUMMARY/ABSTRACT
Broadly neutralizing antibodies are likely to be an important component of an HIV-1 vaccine. The HIV-1 Envelope
(Env) glycoprotein is the sole target of neutralizing antibodies and has been a focus of immunogen designs. Yet
diverse recombinant (rec) Envs do not display detectable binding to the inferred germline (gl) of certain bNAbs,
such as the VRC01-class bNAbs, which are the focus of our IPCAVD project. Rec Envs capable of binding
glVRC01 bNAbs have been designed and induced the production of glVRC01 bNAbs when used as
immunogens. In this IPCAVD, we propose to determine if self-amplifying (sa) mRNA expressing our germline-
targeting 426c.Mod.Core prime and HxB2.WT.Core boost immunogens, expressed either as secreted
nanoparticles (-C4b) or membrane-anchored (gp160ΔCT), will initiate the maturation of VRC01 B cell response
in humans. The Scientific Core Two will provide high quality reagents to Scientific Projects One and Two and
Scientific Core One of this IPCAVD as well as structural information on selected vaccine-elicited Mab of interest
in complex with their antigens, to delineate their epitopes. This information will help us to better understand the
B cell response elicited by the saRNA constructs compared to rec Env immunogens.

## Key facts

- **NIH application ID:** 10771239
- **Project number:** 5U19AI174242-02
- **Recipient organization:** FRED HUTCHINSON CANCER CENTER
- **Principal Investigator:** Marie Pancera
- **Activity code:** U19 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $493,694
- **Award type:** 5
- **Project period:** 2023-01-30 → 2027-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10771239

## Citation

> US National Institutes of Health, RePORTER application 10771239, Scientific Core Two (5U19AI174242-02). Retrieved via AI Analytics 2026-08-17 from https://api.ai-analytics.org/grant/nih/10771239. Licensed CC0.

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