# Intestinal Dysbiosis Exacerbates Inflammation after Cardiopulmonary Bypass in Congenital Heart Disease

> **NIH NIH P20** · UNIVERSITY OF NEBRASKA MEDICAL CENTER · 2024 · $305,395

## Abstract

PROJECT SUMMARY/ ABSTRACT: SALOMON PROJECT
 The intestinal microbiome plays a significant role in maintenance of health and homeostasis. Many disease
states are associated with an altered microbiome, known as dysbiosis, which could exacerbate disease and
worsen inflammation. Previous data has shown children with congenital heart disease (CHD) have an
abnormal intestinal microbiome with increased amounts of proinflammatory bacteria and reduced numbers of
healthy beneficial bacteria. These children also undergo cardiac surgery with cardiopulmonary bypass, which
is known to cause significant amounts of systemic inflammation and induce intestinal epithelial barrier
dysfunction. The systemic inflammation results in low cardiac output state, which is a major contributor to
morbidity and mortality in this patient population.
 We hypothesize that pre-operative intestinal dysbiosis in pediatric patients with CHD produces
inflammatory metabolites that exacerbate CPB-induced intestinal epithelial barrier dysfunction and
systemic inflammation. Our specific aims will map the intestinal microbiome and evaluate intestinal
metabolite profiles associated with inflammatory signaling mediated through intestinal barrier dysfunction. We
will also implement an established piglet model of bypass to evaluate the influence of the microbiome, barrier
function, and metabolites on systemic inflammation. Innovation of this study lies in the concept of studying the
microbiome in patients with congenital heart disease, biological innovation in being the first to temporally map
the microbiome in a variety of cardiac lesions, and technical in the use of an animal model of cardiopulmonary
bypass to evaluate the microbiome.
 This proposal will yield novel evidence of the role the intestinal microbiome plays in relation to congenital
heart disease and the systemic inflammation and patient outcomes following cardiac surgery with
cardiopulmonary bypass. Combined, these results will pave the way for future studies targeting interventions
on the microbiome to improve patient outcomes following surgery with cardiopulmonary bypass.

## Key facts

- **NIH application ID:** 10771700
- **Project number:** 1P20GM152326-01
- **Recipient organization:** UNIVERSITY OF NEBRASKA MEDICAL CENTER
- **Principal Investigator:** Jeffrey D Salomon
- **Activity code:** P20 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $305,395
- **Award type:** 1
- **Project period:** 2024-01-01 → 2028-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10771700

## Citation

> US National Institutes of Health, RePORTER application 10771700, Intestinal Dysbiosis Exacerbates Inflammation after Cardiopulmonary Bypass in Congenital Heart Disease (1P20GM152326-01). Retrieved via AI Analytics 2026-06-24 from https://api.ai-analytics.org/grant/nih/10771700. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*