# CRISPR-Image Core

> **NIH NIH P20** · SOUTH DAKOTA STATE UNIVERSITY · 2024 · $400,396

## Abstract

Project Summary – Core C CRISPR-IMAGE
CRISPR technologies are revolutionizing and accelerating discovery in bioscience. They enable efficient
editing, insertion and deactivation of genes in mammalian cells. Likewise, high-resolution and high-throughput
optical imaging enables rapid analysis of cellular and molecular phenotypes. This core will leverage our recent
advances in CRISPR and optical imaging capabilities along with our extensive experience in fluorescent
protein molecular biology, to create a powerful new resource, embodied within the CRISPR-IMAGE Core.
Here, we propose to unite existing resources with new ones to provide the needed capabilities for this COBRE
(BioSNTR-II). The CRISPR-IMAGE Core will enable cutting-edge genotype-to-phenotype analysis within the
research projects of BioSNTR-II. Specifically, this core will enable creation of the targeted gene knockouts in
cell lines and primary cells proposed in all three of the research projects. The CRISPR-IMAGE Core has three
specific aims: 1) CRISPR-mediated gene disruption and fluorescent protein chimera expression in immune and
inflammatory cells; 2) High-resolution imaging of cellular structures in normal or CRISPR-edited cells; and 3)
High-content microscopy, flow cytometric analysis, and whole-genome CRISPR screening of cellular functions
in gene-edited cells. This core is particularly significant because it brings leading-edge molecular biology and
microscopy technologies to the research projects to help them make potent impacts in biomedical research
and to compete in the national and international funding arenas. This core is highly innovative in that it directly
combines CRISPR gene-editing with optical microscopy methods thereby linking genome-to-phenome
approaches into a compressive workflow.

## Key facts

- **NIH application ID:** 10772050
- **Project number:** 5P20GM135008-03
- **Recipient organization:** SOUTH DAKOTA STATE UNIVERSITY
- **Principal Investigator:** Adam David Hoppe
- **Activity code:** P20 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $400,396
- **Award type:** 5
- **Project period:** 2022-03-20 → 2027-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10772050

## Citation

> US National Institutes of Health, RePORTER application 10772050, CRISPR-Image Core (5P20GM135008-03). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10772050. Licensed CC0.

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