# Immune Phenotyping Core

> **NIH NIH U19** · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · 2024 · $548,082

## Abstract

SUMMARY
The goal of the Immune Phenotyping Core (Core C) is to provide tools and reagents to the different projects
that will be used to characterize the immune responses induced by vaccinations and infections with
coronaviruses, influenza viruses and dengue viruses. The Core will leverage existing state-of-the-art
serological techniques established in the Krammer laboratory, as well as multiplex analysis of cytokine and
chemokine plasma profile and Cytek Aurora Spectral Flow Cytometry profiling of PBMCs and human tonsillar
histocultures (HC) currently used and optimized in the Fernandez-Sesma laboratory. The following aims re
proposed: Aim 1: Characterization of antibody responses to coronavirus, influenza virus and dengue
virus vaccination and infection. The Core will provide assays, reagents and protocols to measure binding
and functional antibody responses against SARS-CoV-2 for Project 1 and influenza viruses for Project 2.
Additionally, the secretion of IgM, IgG and IgA in the supernatant of human tonsil histocultures (HC) treated
with the different SARS-CoV-2 vaccines types (Project 1), influenza virus vaccines and viruses (Project 2) will
be assessed. Aim 2: Characterization of cytokine/chemokine responses to coronavirus, influenza virus
and dengue virus vaccination and infection. The Core will analyze the levels of cytokines and chemokines
in the plasma of vaccinated/infected individuals and the supernatant of human tonsil histocultures treated with
different vaccines for Projects 1, 2 and 3. Aim 3: Characterization of cellular responses to coronavirus,
influenza virus and dengue virus vaccination and infection. Analysis of the cellular immune profiles of
PBMCs from vaccinated/infected individuals over time, using Spectral Flow cytometry. Human tonsillar HC will
be also analyzed by Cytek Aurora Spectral Flow Cytometry in order to capture early immune signatures and
changes in cell populations corresponding to adaptive immune responses in those HC after treatment with
different vaccines. We will obtain high-resolution data at the single-cell level to resolve the most challenging
cell populations including cells expressing viral antigens. PBMCs will also be subjected to a complementary
transcriptomics analysis by RNAseq conducted by the Genomics Core. These tools will serve to generate
immune signatures representative of the longitudinal immune responses to vaccination and/or infection in
study participants enrolled in observational non-interventional cohort studies in coordination with the Data
Management and Analysis Core (Core E). Data obtained using these immunological techniques will be
analyzed by the Data management and Analysis Core comparing them across the different systems used in
the projects as well as in combination with the genomic data obtained in the Genomics Core (Core D) from
the same samples. All data generated by the VIVA Projects and Cores, including the Immune Phenotyping
Core, will be deposited by the Data Analysis ...

## Key facts

- **NIH application ID:** 10783780
- **Project number:** 5U19AI168631-03
- **Recipient organization:** ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
- **Principal Investigator:** Ana Fernandez-Sesma
- **Activity code:** U19 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $548,082
- **Award type:** 5
- **Project period:** 2022-03-22 → 2027-02-28

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10783780

## Citation

> US National Institutes of Health, RePORTER application 10783780, Immune Phenotyping Core (5U19AI168631-03). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10783780. Licensed CC0.

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