Project Summary: The Drosophila Genomics Resource Center (DGRC) supports the international community of scientists utilizing Drosophila melanogaster for biomedical research. The mission of the DGRC is to 1) provide broad access to genomics resources by acquiring, authenticating, archiving, curating, and distributing genomics resources including clones, vectors, and cell lines; 2) facilitate effective use of these genomics resources by providing guidance and support; and 3) improve the genomics resources and protocols available for Drosophila research. By preserving vital research materials and distributing them efficiently, the DGRC assures economical access to important research reagents, and enhances scientific rigor and reproducibility. The first aim of this proposal is to continue to strengthen the successful DGRC programs by: A) Continue to acquire, archive, curate, and distribute Drosophila resources including clones, vectors, and cell lines for supporting fundamental research of biological processes and modeling of aspects of human disease. B) Pilot a program with Indiana University Clinical and Translational Science Institute (CTSI) to promote Drosophila genomics reagents for use in non-Drosophila labs; C) Manage the cost recovery program and resource center to maximize the long-term viability of the DGRC and the benefits to its users and the NIH. The second aim is to improve and update our curation process, data management systems and web interface, and incorporate FAIR and TRUST guiding principles into our reagents, metadata and linking data to other repositories. The goal is to maximize the long-term viability of the DGRC and its benefits to users and the NIH. The third aim is to increase the utility of Drosophila as a model system by generating new resources and protocols through four projects: A) Increase the authentication, and reproducibility and quality control of DGRC reagents; B) Generate gut derived cell lines and pilot and expand to generate a neuroblast derived cell line; C) Continue to expand the catalog of recombination mediated cassette exchange compatible stable attP Drosophila cell lines; D) Collaborate to develop metabolic sensors and standards for aerobic glycolysis and other biosynthetic pathways.