# Regulation of transcriptional activation by protein disorder

> **NIH NIH R01** · UNIVERSITY OF SOUTH FLORIDA · 2023 · $51,191

## Abstract

Title: Acquisition of a fluorescent plate reader for measuring protein-protein and protein-
DNA binding affinities
Summary
Understanding protein-protein and protein-DNA binding is required for a deep understanding of
protein function and its relation to disease. Fluorescence polarization (FP) is an outstanding tool
for measuring protein-protein and protein DNA binding affinities, and it is more flexible than
isothermal titration calorimetry (ITC). Modern FP instruments can measure binding affinities
whether heat is produced and use a microplate reader for high throughput and rapid detection.
We request funds for the Agilent BioTek Synergy H1 microplate reader to improve our ability to
measure binding affinity between p53 mutants and DNA over a range of salt concentrations where
a heat signal from ITC cannot be detected. Results from these experiments will improve our
understanding of how p53 DNA binding specificity is regulated by an intramolecular interaction
between the transactivation and DNA binding domains, which is one of the main goals of the
parent award (R01GM115556).

## Key facts

- **NIH application ID:** 10794041
- **Project number:** 3R01GM115556-08S1
- **Recipient organization:** UNIVERSITY OF SOUTH FLORIDA
- **Principal Investigator:** JIANDONG CHEN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2023
- **Award amount:** $51,191
- **Award type:** 3
- **Project period:** 2016-06-01 → 2026-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10794041

## Citation

> US National Institutes of Health, RePORTER application 10794041, Regulation of transcriptional activation by protein disorder (3R01GM115556-08S1). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10794041. Licensed CC0.

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