Supplement Summary Administrative supplement application for equipment purchase This is an administrative supplement application for the purchase of a AKTA_Pure Fast Pressure Liquid Chromatography (FPLC) system from Cytiva. The AKTA_Pure FPLC is a medium flow, medium pressure chromatography system that allows the semi-automated, rapid, and high-resolution chromatographic separation of proteins by ion-exchange, hydrophobic interaction, gel-filtration, and affinity chromatography. Protein purification and analysis, enabled by the FPLC, is a central and indispensable requirement for all the projects being pursued in my laboratory as part of my NIGMS-funded R35/MIRA award. The new AKTA_Pure FPLC being requested in this supplement will directly replace a malfunctioning AKTA_Explorer instrument that I purchased 13 years ago (in 2009) when I first started my laboratory at Stanford and that is no longer serviceable. We provide documentation from the current vendor (Cytiva) that support for this old AKTA_Explorer system lapsed in 2018. Parts are not available, vendor support is not available and the operating system required to run instrument software is not supported by either Microsoft or Cytiva. Our intensive use (~20-30 hours per week) and project-specific configurations preclude the cost-effective and time-efficient use of chromatography systems in core facilities at Stanford. Finally, the improved automation and software that comes with the new system will dramatically accelerate our workflows, improve reproducibility and provide up-to-date training in the core skills of protein purification and analysis for trainees working on our NIGMS-funded projects. The Biochemistry Department at Stanford will support this purchase with both funds for instrument service and space to house the AKTA_Pure FPLC system.