# Substrate targeting mechanism of a DesCEND pathway

> **NIH NIH R01** · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · 2023 · $51,950

## Abstract

Abstract
This request for a supplement to the grant R01GM135592 proposes the acquisition of an automated high-
throughput crystallization screening system to support the research aims that focus on investigating the substrate
targeting mechanisms of the F-box subunit FBXO31 of the SCF ubiquitin ligase in a novel DesCEND pathway.
By identifying and characterizing substrates of FBXO31 at both a systems level and the molecular level, we
proposed to elucidate the complementary structural determinants of the FBXO31–degron pair that confer
substrate specificity and dictate protein recognition and fate. To achieve our goals, it is vital that we have the
ability to screen wide ranges of crystallization conditions that are essential for obtaining high resolution protein-
substrate complex structures. This proposal requests funds to replace a 10-year old Gryphon-100ul Flex
instrument with a similar but up-to-date version from the same manufacturer. In addition to having reputable
performance quality, the proposed system covers a broad range of screening applications and meets the
automation requirements needed to develop a streamlined approach for crystallization in a high-throughput
fashion. We have obtained institutional support from the Department of Molecular Biology and Biophysics. The
instrument will ensure the continued biochemical, biophysical and structural studies proposed in the parent
award.

## Key facts

- **NIH application ID:** 10797111
- **Project number:** 3R01GM135592-04S1
- **Recipient organization:** UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
- **Principal Investigator:** Bing Hao
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2023
- **Award amount:** $51,950
- **Award type:** 3
- **Project period:** 2020-08-15 → 2026-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10797111

## Citation

> US National Institutes of Health, RePORTER application 10797111, Substrate targeting mechanism of a DesCEND pathway (3R01GM135592-04S1). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10797111. Licensed CC0.

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