# Administrative supplement to purchase a MerMade 4 oligonucleotide synthesizer for the large-scale production of modified RNA substrates

> **NIH NIH R35** · UNIVERSITY OF DELAWARE · 2023 · $95,073

## Abstract

ABSTRACT:
Our NIH R35 ESI MIRA entitled ‘Selectivity and regulation of mRNA demethylation by iron-
dependent dioxygenases’ focuses on understanding the structural and molecular determinants of
AlkBH-family RNA demethylase enzymes that play key roles in RNA function and some human
cancers. A critical challenge to carrying out some of the proposed structural experiments has
been in obtaining sufficient quantities of modified RNA oligonucleotide substrates for
crystallographic screening and biophysical binding assays. This administrative supplement
requests funds to purchase an LGC MerMade 4 oligonucleotide synthesizer that will transform
and greatly accelerate our ability to access key modified RNA oligonucleotide reagents to support
multiple goals of our MIRA projects.

## Key facts

- **NIH application ID:** 10797873
- **Project number:** 3R35GM143000-03S1
- **Recipient organization:** UNIVERSITY OF DELAWARE
- **Principal Investigator:** Jeffrey Scott Mugridge
- **Activity code:** R35 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2023
- **Award amount:** $95,073
- **Award type:** 3
- **Project period:** 2021-08-01 → 2026-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10797873

## Citation

> US National Institutes of Health, RePORTER application 10797873, Administrative supplement to purchase a MerMade 4 oligonucleotide synthesizer for the large-scale production of modified RNA substrates (3R35GM143000-03S1). Retrieved via AI Analytics 2026-06-13 from https://api.ai-analytics.org/grant/nih/10797873. Licensed CC0.

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