# Mechanisms of Regulation of the m6A mRNA Methylation Machinery

> **NIH NIH R35** · YALE UNIVERSITY · 2023 · $214,592

## Abstract

BUDGET JUSTIFICATION
We request the digital PCR Absolute Q from Applied Biosystems to perform all the experiments related to 
assessing in-house gene expression. We use facilities at Yale to perform RNA-seq experiments. These 
experiments, which represent most of the experiments performed in our lab, complement the RNA-sequencing 
experiments and are indispensable to validating and testing all subsequent hypotheses. However, to validate 
and test other experiments, such as m6A-IP followed by qPCR analysis, we currently use our colleague’s qRT-PCR machine, the only one in our institute of seven laboratories. Thus, there is high demand for the instrument,
and it is not always available. Moreover, there is a need to access better technologies, such as digital PCR, that 
allow for the quantitation of RNA molecules without the need for a reference transcript. This grant opportunity 
provides the best option to increase our productivity and the quality of our results.
Acquiring this type of equipment using our current R35 is prohibited since we don’t have a budget reserved for 
such kind of equipment. However, we have part of the R35 budget for small equipment and supplies. We plan 
to use the equipment budget to pay for services and supplies.
The acquisition of this equipment will benefit our lab and our institute since we share most of our equipment. 
My postdocs Marcelo Perez and Swapna Naik have experience with similar equipment, so they can exploit this 
new technology and take care of its maintenance.

## Key facts

- **NIH application ID:** 10798700
- **Project number:** 3R35GM138185-04S1
- **Recipient organization:** YALE UNIVERSITY
- **Principal Investigator:** Claudio R Alarcon
- **Activity code:** R35 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2023
- **Award amount:** $214,592
- **Award type:** 3
- **Project period:** 2020-08-14 → 2025-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10798700

## Citation

> US National Institutes of Health, RePORTER application 10798700, Mechanisms of Regulation of the m6A mRNA Methylation Machinery (3R35GM138185-04S1). Retrieved via AI Analytics 2026-06-24 from https://api.ai-analytics.org/grant/nih/10798700. Licensed CC0.

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