# in situ Epigenetic Profiling of Single Cells in Kidney

> **NIH NIH R21** · UNIVERSITY OF WASHINGTON · 2024 · $185,648

## Abstract

Project Summary/Abstract
The goal of this proposal is to develop and validate two tools for in situ analysis of epigenetic state and
chromatin organization in single cells in kidney tissue. The first tool is based on our recently developed
technology SCEPTRE (Single-Cell Evaluation of Post TRanslational Epigenetic encoding) established for
cultured cells that will be adapted for use with kidney tissues in order to directly resolve and quantify histone
modifications at specific DNA loci in situ in single cells. The second tool is a new method called APT-FISH
(Amplified Probes from Tagmentation Fluorescence in situ Hybridization) that is designed to profile changes to
open chromatin. Each of these tools will be used concurrently with super-resolution expansion microscopy
imaging in order to directly relate nanoscale renal physiology to chromatin or epigenetic state and they will be
applied to the study of aging and senescence in kidney tissue.

## Key facts

- **NIH application ID:** 10802382
- **Project number:** 5R21DK136026-02
- **Recipient organization:** UNIVERSITY OF WASHINGTON
- **Principal Investigator:** Joshua Vaughan
- **Activity code:** R21 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $185,648
- **Award type:** 5
- **Project period:** 2023-04-01 → 2026-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10802382

## Citation

> US National Institutes of Health, RePORTER application 10802382, in situ Epigenetic Profiling of Single Cells in Kidney (5R21DK136026-02). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10802382. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*