# Characterization of the role of the amygdala in reproductive function

> **NIH NIH R01** · BRIGHAM AND WOMEN'S HOSPITAL · 2024 · $449,854

## Abstract

ABSTRACT
Successful reproductive function depends on the proper activation of the gonadotropic axis and
the development of adequate sexual behavior. While the hypothalamic mechanisms regulating
gonadotropin release have been extensively studied, those regulating sexual behavior and
specifically those connecting behavior and fertility have remained elusive. The amygdala has
been documented to integrate sexual cues, e.g. pheromones, to elicit sexual behavior and lesion
studies have indicated its relevance for proper GnRH release in both sexes. Interestingly, the
hypothalamic neuropeptide, kisspeptin (Kiss1), critical for reproductive function, has also been
identified in the amygdala. Our preliminary data documents that the activation of Kiss1 neurons
in the medial amygdala (MeAKiss1 neurons) stimulates LH release. Therefore, our overarching
aim is to characterize in detail the role of MeAKiss1 neurons in males and females in the control of
gonadotropin release and sexual behavior through a combination of functional, pharmacological
and genetic studies that will include: 1) optogenetic and chemogenetic manipulation of cre-
expressing neurons in Kiss1-cre mice; 2) ablation of MeAKiss1 neurons using cre-dependent,
apoptosis inducing, Casp3 viruses; and 3) track tracing of projections from MeAKiss1 neurons to
hypothalamic neurons using fiber markers (mCherry), mono-synaptic track tracers and calcium
photometry circuit mapping. Moreover, we will characterize the interaction between these two
functions (i.e. gonadotropin release and sexual behavior) by assessing the activation of Kiss1 and
GnRH neurons during mating, and the activation of hypothalamic nuclei involved in sexual
behavior during the LH surge in females. Overall, the successful completion of this project will
significantly extend our understanding of the role of the medial amygdala in the integration of
sexual behavior and gonadotropin release, which is fundamental to synchronize mating and
ovulation for successful reproduction. The expected findings will offer new platforms for the
treatment of reproductive disorders of central origin such as hypogonadism or low sex drive.

## Key facts

- **NIH application ID:** 10805434
- **Project number:** 5R01HD099084-05
- **Recipient organization:** BRIGHAM AND WOMEN'S HOSPITAL
- **Principal Investigator:** Victor Manuel Navarro
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $449,854
- **Award type:** 5
- **Project period:** 2020-04-22 → 2026-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10805434

## Citation

> US National Institutes of Health, RePORTER application 10805434, Characterization of the role of the amygdala in reproductive function (5R01HD099084-05). Retrieved via AI Analytics 2026-06-11 from https://api.ai-analytics.org/grant/nih/10805434. Licensed CC0.

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