# Using Directed Evolution to Srudy the Origins of Multicellular Development

> **NIH NIH R35** · GEORGIA INSTITUTE OF TECHNOLOGY · 2023 · $15,375

## Abstract

Grant R35GM138030 utilizes directed evolution to study the evolution of multicellularity in the 'snowflake
yeast' model system. The proposed work involved using single cell RNA-seq to examine the origin of multiple
cell types over ~5,000 generations of evolution. Snowflake yseat evolve three distinct cell states, with elevated
gene expression related to ribosomal processes, cell wall biogenesis, and stress response and programmed cell
death, respectively. During a recent internship, undergraduate Luis Felipe Cedeno Perez developed a method to
measure real-time single-cell phenotypic data in developing snowflake yeast clusters via soft-agar
immobilization and image segmentation. He used machine learning to construct cell fate maps. His internship
will end in April, 2023. If funded, Luis Felipe's summer project will allow him to complete his project, by
integrating differential gene/protein expression into his segmentation pipeline, allowing for the construction of
cell fate lineage maps and examination of location-dependent gene expression during ontogeny.
Luis Felipe will gain expertise in experimental procedures and computational approaches, working closely with
a postdoc in the lab for in-lab mentoring as well as meeting regularly with the PI. He will also participate in
activities to improve his project management, scientific communication, and teamwork skills. The supplement
will enable Luis Felipe to finish his project and generate the data required for a first-author paper, making him
competitive for top-tier PhD programs in biomedical research. If he joins the Ratcliff lab as a PhD student, the
project will give him a head start on his PhD thesis research and make him more competitive for proposals that
would fund his PhD research.

## Key facts

- **NIH application ID:** 10807198
- **Project number:** 3R35GM138030-03S1
- **Recipient organization:** GEORGIA INSTITUTE OF TECHNOLOGY
- **Principal Investigator:** William Croft Ratcliff
- **Activity code:** R35 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2023
- **Award amount:** $15,375
- **Award type:** 3
- **Project period:** 2020-08-01 → 2025-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10807198

## Citation

> US National Institutes of Health, RePORTER application 10807198, Using Directed Evolution to Srudy the Origins of Multicellular Development (3R35GM138030-03S1). Retrieved via AI Analytics 2026-06-02 from https://api.ai-analytics.org/grant/nih/10807198. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*