Abstract Dr. Greenhouse is a physician scientist trained in internal medicine, infectious diseases, and biostatistics. Over the past 18 years, he has worked across domains of field epidemiology, laboratory science, data science, and public health to investigate the epidemiology of infectious diseases, with a focus on disease transmission and development of immunity. His growth as a scientist and mentor was substantially accelerated due to the dedicated time afforded by his current K24. Studies to accomplish the research aims outlined in his prior K24 application have all been published as peer-reviewed papers and/or are the subject of active, R01- level NIH grants where he or a clinician scientist that he mentors serves as principal investigator. During this project period he published 80 papers, was PI on 3 new grants including R01 and U01 awards from NIAID, was co-investigator on 6 new grants, and was primary mentor for 2 new K awards. Regarding mentorship, he supervised 39 trainees including 14 from low- or middle-income countries, cofounded the UCSF EPPIcenter as a center of excellence for research and training in patient-oriented research, created two longitudinal training programs in malaria genetic epidemiology for African scientists, and co-led 3 workshops in genomic epidemiologic analysis for over 100 African scientists. During the next K24 project period, Dr. Greenhouse proposes to 1) focus on leadership development so he can better guide the growth and success of the EPPIcenter; 2) expand the scope of his research portfolio to include pathogens beyond malaria and the addition of domestic research; and 3) establish an interactive, online training platform in genomic epidemiologic analysis. He presents a plan for achieving these goals by obtaining leadership development training, expanding his skill set in the epidemiology of respiratory viruses and laboratory methods for multi-pathogen surveillance, and developing expertise in curricular development and other principles such as the use of technology for effective teaching. Dr. Greenhouse’s existing research portfolio provides a wealth of opportunities for trainees, including projects studying the longitudinal development of naturally acquired immunity to malaria using birth cohorts to simultaneously study the pathogen and the host; systems immunology and computational analysis to comprehensively characterize factors associated with development of immunity to malaria; and an array of projects related to the genomic epidemiology of malaria including multiple with explicit training and capacity building goals. He proposes to expand the scope of his research portfolio with 3 specific aims: 1) to develop and apply amplicon sequencing tools to solve current challenges in malaria surveillance; 2) to characterize the pathogen landscape of non- malarial febrile illness in Uganda; and 3) to characterize the transmission of respiratory pathogens in an urban immigrant community in San Francisco.