# Investigating the effect of alcohol exposure on human cortical development using a 3D in vitro model

> **NIH NIH F32** · CHILDREN'S RESEARCH INSTITUTE · 2023 · $1,221

## Abstract

Project summary
This project is prepared in response to PA-20-272 (Administrative Supplements to Existing NIH Grants and
Cooperative Agreements (Parent Admin Supp Clinical Trial Optional). The PI is in no cost extension period
of her F32 fellowship, partially supported by funds carried forward from previous years. The PI successfully
generated human iPSC-derived cortical spheroids in defined, differentiation-promoting culture medium and
investigated the effect of ethanol on early brain development. We have assessed the effects of ethanol on
cortical development by monitoring cell death, morphological change, cellular proliferation, and the
progenitor cell population with an emphasis on outer radial glia-like cells in the spheroids at cellular level.
This proposed administrative supplement will allow the PI completing analysis of acquired RNA sequencing
data in preparation of future manuscript. This administrative supplement will also provide the necessary
resources allowing an orderly completion of the parent project.

## Key facts

- **NIH application ID:** 10811223
- **Project number:** 3F32AA028163-03S1
- **Recipient organization:** CHILDREN'S RESEARCH INSTITUTE
- **Principal Investigator:** Ray Yueh Ku
- **Activity code:** F32 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2023
- **Award amount:** $1,221
- **Award type:** 3
- **Project period:** 2023-03-17 → 2023-09-29

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10811223

## Citation

> US National Institutes of Health, RePORTER application 10811223, Investigating the effect of alcohol exposure on human cortical development using a 3D in vitro model (3F32AA028163-03S1). Retrieved via AI Analytics 2026-05-26 from https://api.ai-analytics.org/grant/nih/10811223. Licensed CC0.

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