# Clonal hematopoiesis and inherited genetic variation in sickle cell disease

> **NIH NIH R01** · VANDERBILT UNIVERSITY MEDICAL CENTER · 2024 · $764,009

## Abstract

Project Summary
Sickle cell disease (SCD) is associated with chronic hemolysis, systemic endothelial dysfunction,
inflammation and vascular occlusion. This complex pathophysiology leads to severe pain,
progressive multi-organ damage and premature death with a median lifespan of 48 years in high-
income countries. We and others have determined that young adults with progressive heart, lung,
and kidney damage, either individually or in combination, are at particularly high risk for premature
death. Many individuals with SCD are candidates for high-risk treatments that can potentially
eliminate symptoms and arrest organ damage, including allogeneic hematopoietic stem cell
(HSC) transplantation and various forms of autologous HSC gene therapy. However, several
individuals who received these treatments have developed acute myeloid leukemia or other
myeloid neoplasms. In many cases, the blood cancer arose from an autologous, premalignant
HSC harboring a somatic “clonal hematopoiesis” (CH) mutation that was present before therapy.
We and others have shown in individuals without SCD that CH mutations confer a growth
advantage to aging HSCs, predisposing to not only myeloid leukemia, but also endovascular
disease affecting the heart, lung and kidney. Additional preliminary data derived from deidentified
genomic or exonic sequences indicate that individuals with SCD develop CH at earlier ages than
that of the general population. Based on these data, we hypothesize that individuals with SCD
have an increased prevalence of CH, which accelerates the development of heart, lung,
and kidney disease. We will test this hypothesis by first determining the prevalence and
incidence of CH in three well-characterized multi-center cohorts of older children and adults with
SCD (n= 2645) and matched controls (n= 7935, Aim 1). We will use a novel, scalable, cost-
effective, error-corrected sequencing assay that can detect low-level (0.1%) somatic CH
mutations. Next, we will determine whether CH mutations are associated with heart, lung or
kidney disease in these cohorts (Aim 2). Our team has already completed whole-genome
sequencing of the cohorts through the NIH NHLBI Trans-Omics for Precision Medicine (TOPMed)
program, which will allow us to study genetic interactions between CH mutations and germline
variants that are known to influence SCD outcomes. Our project will provide novel insights into
the importance of CH as a risk factor for heart, lung, and kidney disease in SCD, identify
individuals who could benefit from individualized strategies for organ protection administered
prospectively, and fuel future studies to determine whether CH predisposes to the development
of myeloid leukemia after allogeneic HSC transplantation or gene therapy for SCD.

## Key facts

- **NIH application ID:** 10812435
- **Project number:** 5R01HL168179-02
- **Recipient organization:** VANDERBILT UNIVERSITY MEDICAL CENTER
- **Principal Investigator:** Alexander Bick
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $764,009
- **Award type:** 5
- **Project period:** 2023-04-01 → 2027-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10812435

## Citation

> US National Institutes of Health, RePORTER application 10812435, Clonal hematopoiesis and inherited genetic variation in sickle cell disease (5R01HL168179-02). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10812435. Licensed CC0.

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