# Biophysics Core

> **NIH NIH U54** · SEATTLE CHILDREN'S HOSPITAL · 2024 · $759,060

## Abstract

ABSTRACT, CORE 3
Biophysical methods, such as single-molecule techniques, quantitative imaging/spectroscopy and native mass
spectrometry (nMS) are powerful tools to gain mechanistic insights into key dynamic interactions and processes
occurring during the early and late steps of the HIV-1 lifecycle. The goal of Core 3 (Biophysics Core) is to provide
requisite instrumentation, expertise and training to augment the B-HIVE capabilities across all three center
projects by enabling cutting-edge studies of protein-protein, protein-nucleic acid and protein-membrane
interactions in living cells and in model systems. To accomplish these goals we will: (1) Provide advanced
fluorescence microscopy capabilities to visualize early and late events of the HIV life cycle (Melikian); (2) provide
advanced capabilities in single-molecule biophysics to probe dynamics of proteins, nucleic acids and their
complexes (Millar & Musier-Forsyth); and (3) provide methods for biophysical characterization of
macromolecular complexes (Musier-Forsyth and Wysocki). A critical part of the Core 3 mission is training the
users remotely or in person, through visits to the labs of the Core co-leads, workshops and videoconferences.
To accomplish this goal, the core will provide basic imaging, single-molecule and nMS services using established
pipelines to all B-HIVE projects, as needed, implement advanced techniques and engage in long-term projects
through collaborative efforts with the B-HIVE investigators, and develop novel technologies in response to the
needs of Projects 1-3.
1

## Key facts

- **NIH application ID:** 10812446
- **Project number:** 5U54AI170855-03
- **Recipient organization:** SEATTLE CHILDREN'S HOSPITAL
- **Principal Investigator:** Gregory B Melikian
- **Activity code:** U54 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $759,060
- **Award type:** 5
- **Project period:** 2022-06-22 → 2027-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10812446

## Citation

> US National Institutes of Health, RePORTER application 10812446, Biophysics Core (5U54AI170855-03). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10812446. Licensed CC0.

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