Abstract A key component of Phase III CoBRE funding for the Center for Cancer Biology Research (CCBR) is support of the Flow Cytometry Core (Core B). This core, established in 2011 with the initiation of Phase I, is extensively used by CCBR investigators, those at Sanford Research at large, investigators from regional institutions, and recently by contractual users. The Core provides state-of-the-art equipment for flow cytometry, cell sorting, and related sample preparation. The Flow Core staff provides a wide array of services in terms of instrumentation, training, and experimental design. This allows investigators to obtain high quality data to support publications and grant proposals. For Phase III, the Core presents a feasible plan for transitioning to independence. This plan includes a fee-for-services schedule. Also, the Core will continue to expand its services through the acquisition of state-of-the-art instrumentation and a growing user base, including additional clientele from regional institutions and contractual arrangements. Already, the reputation of the Core's quality work and quick turnaround has expanded regional and contractual usage. Based on this growth and substantial institutional support through personnel salaries and equipment purchases and maintenance, self-sufficiency will be attained. following Phase III support.