# The Organoid and Cell Culture Core

> **NIH NIH P30** · COLUMBIA UNIVERSITY HEALTH SCIENCES · 2024 · $165,030

## Abstract

SUMMARY
The Organoid and Cell Culture Core (OCCC) will contribute to the mission of the Columbia University Digestive
and Liver Disease Research Center (CU-DLDRC) by serving members’ needs related to human and murine cell
culture systems for modeling of gastrointestinal, hepatobiliary and pancreatic diseases. These systems include
primary culture, genetically-engineered cell lines, spheroids and organoids as well as 3D co-culture. OCCC
platforms will benefit the majority of clinical and basic CU-DLDRC investigators, providing powerful tools to study
epithelial cells and their interactions in digestive homeostasis and disease, the central theme of the CU-DLDRC.
Directed by Drs. Hiroshi Nakagawa, MD, PhD and Kelley Yan, MD, PhD, two scientists with long-standing
complementary expertise in advanced cell culture and organoids, the OCCC will provide services, technologies,
quality control and cost-effectiveness to the CU-DLDRC investigators, including the Pilot and Feasibility grant
recipients. The OCCC will provide a rich repository of cell lines and human and mouse organoids that are well
annotated for identity, passage number, and Mycoplasma infection-free status, thereby providing quality control,
rigor and reproducibility. The OCCC will also assist CU-DLDRC investigators to generate 3D organoids from
their human and mouse tissues as well as from inducible pluripotent stem (iPS) cells, to analyze growth,
morphology, gene expression, and cell-cell interactions via co-culture experiments. The OCCC will integrate
advanced cell culture technologies for manipulating genome and gene expression via CRISPR/Cas9, RNA
interference, and inducible retroviral/lentiviral gene expression vectors in cell and organoid-based systems.
These approaches are further enhanced by coordinated multi-core workflows with the Clinical Biospecimen and
Research Core for patient- and disease-specific organoids; with the Bioinformatics and Single Cell Analysis Core
for the plate-RNAseq-based CRISPR and drug screens; and with the Bioimaging Core for functional visualization
of cells and organoids. The OCCC will promote the mission of the CU-DLDRC through the following interrelated
Specific Aims: To provide CU-DLDRC members and their laboratories with cell lines, advanced cell culture
platforms and technologies (Aim 1); to build and expand unique CU-DLDRC and national repositories of disease-
specific human and mouse 3D organoid libraries (Aim 2); and to enhance the technical capabilities of new and
established CU-DLDRC investigators through training on cell and organoid culture models and as well as
educational seminars on novel developments (Aim 3). The OCCC will be highly utilized with 71% of CU-DLDRC
members indicating use, most of them use of multiple OCCC services. In summary, the OCCC will provide highly
efficient and cost-effective services and unique models that do not currently exist in CU-DLDRC members’
laboratory and therefore constitute significant benefits. Through it...

## Key facts

- **NIH application ID:** 10818425
- **Project number:** 5P30DK132710-03
- **Recipient organization:** COLUMBIA UNIVERSITY HEALTH SCIENCES
- **Principal Investigator:** Hiroshi Nakagawa
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $165,030
- **Award type:** 5
- **Project period:** 2022-04-30 → 2027-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10818425

## Citation

> US National Institutes of Health, RePORTER application 10818425, The Organoid and Cell Culture Core (5P30DK132710-03). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/10818425. Licensed CC0.

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