# Single cell tri-omics: High coverage simultaneous profiling of DNA, RNA, and proteins in single cells

> **NIH NIH R43** · VIVID BIOINNOVATIONS, PBC · 2024 · $268,142

## Abstract

ABSTRACT
Alzheimer's disease, a devastating neurologic disorder, accounts for 60-80% of all dementias, making the
development of new technologies for studying the disease crucial. Fluid Discovery aims to advance single-cell
proteomics with a groundbreaking product called Self-Assembled Partitioning Proteomics (SAP-pm), which will
quantify thousands of proteins in hundreds of thousands of individual cells and can be multiplexed with single
cell DNA and RNA sequencing. Building on cutting-edge work in microfluidics, biophysics, and genomics, SAP-
pm offers a unique, rapid, and cost-effective method to overcome limitations in protein coverage and sensitivity
found in current technologies. The project comprises two aims: (1) Develop SAP-pm for sensitive, thousand-plex
analysis of proteins at the single cell level, and (2) Expand the capability of SAP-pm to analyze brain nuclei and
simultaneously capture mRNA, facilitating valuable insights from primary human tissues to improve human
health.

## Key facts

- **NIH application ID:** 10821171
- **Project number:** 1R43NS135703-01
- **Recipient organization:** VIVID BIOINNOVATIONS, PBC
- **Principal Investigator:** Robin Prince
- **Activity code:** R43 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $268,142
- **Award type:** 1
- **Project period:** 2024-02-01 → 2026-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10821171

## Citation

> US National Institutes of Health, RePORTER application 10821171, Single cell tri-omics: High coverage simultaneous profiling of DNA, RNA, and proteins in single cells (1R43NS135703-01). Retrieved via AI Analytics 2026-05-26 from https://api.ai-analytics.org/grant/nih/10821171. Licensed CC0.

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