# Redox Regulation of Host Ribosomal Proteins during Helicobacter pylori infection

> **NIH NIH F31** · YALE UNIVERSITY · 2024 · $48,116

## Abstract

PROJECT SUMMARY
Helicobacter pylori infection is commonly associated with several gastric diseases including gastritis, peptic ulcer
disease, lymphoma of the mucosa-associated lymphoid tissue, and gastric adenocarcinoma. H. pylori has
evolved various mechanisms to evade the host innate immune response, and as a result, these bacteria can
thrive for decades in the gastric mucosa. Chronic infection elicits the continuous generation of reactive oxygen
species (ROS) by neutrophils and gastric epithelial cells. Elevated levels of ROS can damage macromolecules,
and previous studies have focused on how ROS mediate DNA damage during infection. However, ROS can also
generate oxidative post-translational modifications on host proteins containing redox-sensitive cysteines that
regulate important cellular functions. Our lab previously performed a chemical proteomic screen to identify
cysteines in host proteins that exhibit decreased reactivity during H. pylori infection of human gastric cancer
cells. This proposal will focus on two ribosomal proteins, uL14 and eS27, which contain cysteine residues that
were among the top hits from this screen. Host translational inhibition is a well-characterized response to
infection by several microbes, and Aim 1 of this proposal will elucidate whether ribosome biogenesis and
translational function are similarly affected during H. pylori infection. Aim 2 will focus on characterizing how the
reactivity of the specific cysteines Cys125 of uL14 and Cys77 of eS27 contributes to the cellular localization and
interactions of the ribosomal proteins. Overall, this proposal will provide insight into how redox regulation of host
ribosomal proteins affects their function and expand our understanding of host cellular processes that are
modulated by H. pylori infection.

## Key facts

- **NIH application ID:** 10823409
- **Project number:** 1F31AI176757-01A1
- **Recipient organization:** YALE UNIVERSITY
- **Principal Investigator:** Renuka Ganesh Ramanathan
- **Activity code:** F31 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $48,116
- **Award type:** 1
- **Project period:** 2024-09-01 → 2026-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10823409

## Citation

> US National Institutes of Health, RePORTER application 10823409, Redox Regulation of Host Ribosomal Proteins during Helicobacter pylori infection (1F31AI176757-01A1). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10823409. Licensed CC0.

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