# Mechanisms and regulation of mRNA 3' processing

> **NIH NIH R35** · UNIVERSITY OF CALIFORNIA-IRVINE · 2023 · $83,407

## Abstract

Project summary:
The long-term goal of my research program is to understand, in detail, the mechanisms of
mammalian mRNA 3’ processing and its regulation. mRNA 3’-end formation, typically
involving an endonucleolytic cleavage followed by polyadenylation, is an essential step of
eukaryotic gene expression and it significantly impacts many aspects of RNA metabolism,
including mRNA stability, subcellular localization and translation. In addition, the majority
of eukaryotic genes produce multiple mRNA isoforms with distinct 3’ ends through
alternative polyadenylation (APA). Recent studies have revealed that APA is highly
regulated in development and plays an important role in post-transcriptional gene
regulation. Aberrant APA patterns have been associated with a wide range of diseases,
from cancer to neurological disorders. Key outstanding questions in the mRNA 3’
processing field include: 1) what is the structure-function relationship of the mRNA 3’
processing complex; 2) how is mRNA 3’ processing regulated by cell signaling; 3) how is
APA regulated by RNA-binding proteins? We will address these questions by using
biochemical, structural, and genomic analyses. The results of these studies will not only
provide novel insights into this key step in eukaryotic gene expression, but also pave the
way for developing novel therapy for many diseases.

## Key facts

- **NIH application ID:** 10824010
- **Project number:** 3R35GM149294-01S1
- **Recipient organization:** UNIVERSITY OF CALIFORNIA-IRVINE
- **Principal Investigator:** Yongsheng Shi
- **Activity code:** R35 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2023
- **Award amount:** $83,407
- **Award type:** 3
- **Project period:** 2023-05-01 → 2024-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10824010

## Citation

> US National Institutes of Health, RePORTER application 10824010, Mechanisms and regulation of mRNA 3' processing (3R35GM149294-01S1). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10824010. Licensed CC0.

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