PROJECT SUMMARY SAMPLE CORE The main function of the Sample Core is to provide Research Projects 1 and 2, and the Technology Development Project with human specimens to support the proposed research of this CCHI. The Core will manage the collection of coded, appropriately consented tissue samples and their distribution. The Core will critically intersect all Projects and Cores of the JAX-ISMMS CCHI program; as such, it will be an integral part of the program in the execution of the proposed research, as well as in the provision of ongoing sample and data management throughout the life of each Project. The Core will: 1) serve as a central resource for CCHI investigators to obtain appropriate lung, blood, bone marrow, microbiome (oral and nasopharyngeal samples for future follow-up studies) and associated demographic data for research purposes; 2) ensure that samples were acquired consistent with all applicable regulations for the use of human tissues in research, including human subjects consent; and 3) ensure that all specimens and associated information generated by each Project will be systematically cataloged, tracked, and stored. The Core will also generate and distribute air-liquid interface (ALI) cultures for experiments. The Core will draw on the extensive expertise of the Core Leader, Dr. Karolina Palucka, and her team in sample procurement and management; on the sample storage and data management infrastructure at The Jackson Laboratory for Genomic Medicine; and on the robust collaborations established with the CCHI's clinical partners at the Hartford HealthCare Cancer Institute at Hartford Hospital (Andrew Salner, M.D., and Peter Yu, M.D.), Nationwide Children's Hospital (Mark Peeples, Ph.D.), and Benaroya Research Institute (Carmen Mikacenic, M.D.). The Specific Aims of the Sample Core are: Aim 1: Oversee sample collection, acquisition, and processing; Aim 2: Manage sample storage, tracking, and distribution; Aim 3: Generate and distribute ALI cultures; and Aim 4: Oversee personnel training and compliance.