# Neural Circuit Dynamics Underlying Perceptual Decision Making in the Macaque Dorsolateral Prefrontal Cortex > **NIH NIH F31** · BOSTON UNIVERSITY (CHARLES RIVER CAMPUS) · 2024 · $38,442 ## Abstract PROJECT SUMMARY/ABSTRACT The objective of the proposed research is to 1) understand if activity of various cell types in DLPFC is consistent with computational models of decision-making, and 2) investigate if there is a functional hierarchy in macaque monkey dorsolateral prefrontal cortex (DLPFC) during decision-making. Decision-making is our ability to choose and perform appropriate actions based on sensory cues and context to achieve behavioral goals. DLPFC is a key decision-making brain region which receives inputs from higher-order sensory areas and sends outputs to premotor cortices. DLPFC is also implicated in disorders of decision-making such as schizophrenia but currently, it is unknown how circuit-level disruptions in this disorder result in its perturbed function. Specifically, we do not understand 1) how different cell types in DLPFC mechanistically enable decision-making and 2) the spatial organization of function in this area. We address these questions by combining in vivo neurophysiology with computational modeling in DLPFC of monkeys performing our novel red-green reaction time checkerboard discrimination tasks. Our first aim tests three emerging hypotheses of the role of cell types during decision- making: 1) are inhibitory cells choice-selective or -nonselective during decision-making? 2) Do inhibitory neurons exhibit contra-specific rather than ipsi-specific connectivity? 3) Do either somatostatin- (SST+) or parvalbumin- positive (PV+) support decision-making through choice-selectivity? In this first aim, we will first build several cell- type specific computational models of decision-making to establish predictions of neural dynamics during the task. We will then use laminar multi-contact electrodes to record single neurons from DLPFC of monkeys performing our novel red-green reaction time checkerboard discrimination task. With these recorded responses, we will adjudicate between the models. Finally, we will examine each unit’s electrophysiological properties (waveform shape and firing rate patterns) with our recently developed and validated approach that allows us to delineate putative cell types (PhysMAP). With these identified populations, we will test predictions made by our cell type-specific mean-field models of decision-making. In our second aim, we will test three competing theories this time regarding the nature of functional gradients in DLPFC: 1) the “modular hypothesis” that different areas are qualitatively distinct in their representations; 2) the “gradient hypothesis” that different areas contain the same information which differs quantitatively in the “amount”; or 3) the “hierarchical hypothesis” that “upstream” areas contain all decision variables whereas "downstream” areas only contain action-oriented variables. We will record from DLPFC across several anatomical axes during two variants of the checkerboard task. We will then use decoding and population analysis approaches like demixed principal component ana... ## Key facts - **NIH application ID:** 10825871 - **Project number:** 1F31NS131018-01A1 - **Recipient organization:** BOSTON UNIVERSITY (CHARLES RIVER CAMPUS) - **Principal Investigator:** Eric Kenji Lee - **Activity code:** F31 (R01, R21, SBIR, etc.) - **Funding institute:** NIH - **Fiscal year:** 2024 - **Award amount:** $38,442 - **Award type:** 1 - **Project period:** 2024-01-01 → 2025-11-30 ## Primary source NIH RePORTER: https://reporter.nih.gov/project-details/10825871 ## Citation > US National Institutes of Health, RePORTER application 10825871, Neural Circuit Dynamics Underlying Perceptual Decision Making in the Macaque Dorsolateral Prefrontal Cortex (1F31NS131018-01A1). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10825871. Licensed CC0. --- *[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*