# Health Effects of the Fluorinated Pollutants; PFAS on Enamel Development

> **NIH NIH K02** · NOVA SOUTHEASTERN UNIVERSITY · 2022 · $58,156

## Abstract

Dr. Suzuki's (PI) ultimate research goal is to identify environmental factors related to craniofacial
pathophysiology and develop novel preventive and therapeutic strategies for environmental factor-associated
oral diseases. This career development K02 award would provide the protected time 1) to gain expertise in
physical analysis of skeletal tissues, including Micro-CT, FIB-SEM and QLF, and 2) to establish collaborative
relationships with experts in environmental health science field. The proposed research project aims to
characterize the health effects of fluorinated pollutants PFAS (per- and polyfluoroalkyl substances or
organofluorine compounds) on tooth development. PFAS are a group of man-made organofluorine
compounds, including Perfluorooctanoic acid (PFOA) and PFOA precursor, Fluorotelomer alcohols (FTOHs).
PFAS do not readily breakdown in the environment and are water-soluble. PFAS can be found in drinking
water and living organisms, including fish, animals and humans where PFAS can build up and persist over
time. Laboratory animal studies showed that PFAS can cause tumors and adverse effects on reproductivity,
development and immune system. Previous studies demonstrated that FTOHs (precursor of PFOA) induced
tooth malformation, including degeneration of ameloblasts in rodent incisors. However, examination of how
FTOHs alter tooth phenotype (physical and histological) is limited and the molecular mechanisms of how
FTOHs affect tooth development are largely unknown. Our long-term goal is to identify the molecular
mechanisms of PFAS adverse effects on odontogenesis. Our overall objective here is to establish PFAS
(hazardous chemical) use in an animal model and determine how FTOHs affect amelogenesis in vivo. Our
central hypothesis is that FTOHs induce DNA damage and mitochondrial damage to perturb ameloblast
function during tooth development that results in enamel malformation. Our hypothesis has been formulated
based on our preliminary data showing that PFOA inhibited cell proliferation, induced apoptosis, DNA damage
and mitochondrial damage in ameloblast-like cell (LS8 cells). The impact of the proposed research is to define
the effects of PFAS on tooth development and to highlight the molecular mechanisms involved in tooth
malformation. Once PFAS adverse effects are identified in tooth formation, PFAS could be considered as a
possible causative factor for cryptogenic abnormalities in dentinogenesis, including Molar Incisor
Hypomineralisation (MIH) of which the etiology is unknown. We plan to test our central hypothesis and
accomplish our overall objective by pursuing the Specific AIM: Identify FTOH effects on enamel phenotype in a
mouse model.

## Key facts

- **NIH application ID:** 10827647
- **Project number:** 7K02DE029531-02
- **Recipient organization:** NOVA SOUTHEASTERN UNIVERSITY
- **Principal Investigator:** Maiko Suzuki
- **Activity code:** K02 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $58,156
- **Award type:** 7
- **Project period:** 2023-04-12 → 2025-09-14

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10827647

## Citation

> US National Institutes of Health, RePORTER application 10827647, Health Effects of the Fluorinated Pollutants; PFAS on Enamel Development (7K02DE029531-02). Retrieved via AI Analytics 2026-05-27 from https://api.ai-analytics.org/grant/nih/10827647. Licensed CC0.

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