# Testing Cerebroprotective Interventions with Rodent Ischemic Stroke Models

> **NIH NIH U01** · UNIVERSITY OF CALIFORNIA, SAN DIEGO · 2024 · $598,262

## Abstract

PROJECT SUMMARY: The objective of this proposal is to test cerebroprotective interventions for the
SPAN program by utilizing an intraluminal middle cerebral artery occlusion (MCAO) mouse model or an
animal blood clot embolic model. Our lab has been using innovative techniques in producing highly consistent
mouse MCAO and clinically relevant animal blood clot embolic stroke models. Our animal surgeons have
more than 15 years of experience in various animal ischemic stroke models and have performed surgeries on
thousands of animals of various species (e.g., mice, rats, and rabbits). We established an easy-to-use
technique to monitor the middle cerebral artery (MCA) blood flow throughout the peri-MCAO periods. Our
animal operating and behavioral exam rooms have about 900 square feet of space and were completely
renovated in 2019. Our three animal surgical stations with matching monitoring instruments are state-of-the-
art. Our Bruker 9.4 T MRI Scanners are advanced and allow for high-resolution quantitative assessment of
CNS structures and functions. Our state-of-the-art infrastructure, together with our highly skilled personnel,
allows us to run multiple studies in parallel. We have about a decade of experience in performing interactive
multi-institutional projects funded by the NIH. We have published more than ten studies about stroke-related
comorbidities. Death and disability/dependency are always key primary outcome measures in stroke clinical
trials. However, many functional tests in animal stroke studies are not designed to assess animal “natural (i.e.,
minimal investigator interaction)” disability; rather, animals are required or forced to perform tasks. These
tasks are highly useful to test specific deficits but may differ from “natural” disability/dependency that presents
in stroke clinical trials. Therefore, we recently established two novel tests to reflect animal long-term “natural”
disability (unable to work, eat, and drink by themselves): (i) nest building activity measuring ability to work,
and (ii) PhenoTyper monitoring the “total” activity, food and drink intake activities, and time of death. These
long-term “natural” behavioral tests are objective, easy-to-use, measurable, and sensitive, and may mimic the
clinically relevant disability/dependency benchmarks used in stroke clinical trials. In the first year, we will: (i)
set up the required infrastructure and animal models; (ii) sign agreements and participate in all SPAN
meetings; (iii) share data with the Coordinating Center (CC); and (iv) execute the animal studies following the
assignments and protocols set forth by SPAN. In the second year, we will further: (i) execute the animal
studies; (ii) present our results to SPAN for recommendations of go/no-go, and (iii) participate in all SPAN
meetings and share resources, infrastructure, and protocols. In the third year, we will continue to execute the
animal studies and participate in all scheduled SPAN meetings. We will consult th...

## Key facts

- **NIH application ID:** 10828812
- **Project number:** 5U01NS130557-02
- **Recipient organization:** UNIVERSITY OF CALIFORNIA, SAN DIEGO
- **Principal Investigator:** Bingren Hu
- **Activity code:** U01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $598,262
- **Award type:** 5
- **Project period:** 2023-04-15 → 2026-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10828812

## Citation

> US National Institutes of Health, RePORTER application 10828812, Testing Cerebroprotective Interventions with Rodent Ischemic Stroke Models (5U01NS130557-02). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/10828812. Licensed CC0.

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