# Core 1 Krogan

> **NIH NIH U54** · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · 2024 · $393,809

## Abstract

THE HARC CENTER: HIV ACCESSORY AND REGULATORY COMPLEXES
PROTEOMICS CORE
SUMMARY
The Proteomics Core will support all HARC Center Projects by applying and developing mass spectrometry
(MS)-based proteomic technologies for the functional and structural characterization of HIV-host protein
complexes. Together with the Genetics, Structural Biology, and Computational Cores, we provide a
systematic pipeline for the structure determination of Vif-, Tat-, and Rev-host protein complexes. This novel
HARC endogenous protein structure (HEPS) platform will collect and integrate structural data to build
comprehensive structural models of endogenously purified virus-host complexes. The Proteomics Core will
perform endogenous protein purifications and map protein-protein interactions (PPIs) of HIV and host proteins
in HIV-infected and genomically edited and unedited primary CD4+ T cells by affinity-purification MS. We will
additionally provide structural proteomics methodologies, including native MS (nM); cross-linking (XL)-MS, and
hydrogen/deuterium exchange-MS (H/DX-MS), to support the structure determination of challenging and flexible
HIV-host protein complexes. Finally, to characterize changes to chromatin and RNA-binding proteins involved in
HIV transcription and latency, we will identify and quantitatively compare post-translational modifications (PTMs)
of wild-type (WT) and genomically modified CD4+ primary cells infected with WT or mutant HIV.

## Key facts

- **NIH application ID:** 10829949
- **Project number:** 5U54AI170792-03
- **Recipient organization:** UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
- **Principal Investigator:** Danielle L Swaney
- **Activity code:** U54 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $393,809
- **Award type:** 5
- **Project period:** 2022-07-15 → 2027-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10829949

## Citation

> US National Institutes of Health, RePORTER application 10829949, Core 1 Krogan (5U54AI170792-03). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10829949. Licensed CC0.

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