The Exposure Signatures Facility Core (ESFC) is a consortium of six analytical resources that each contribute unique capabilities to identifying and quantifying toxicants and biological responses to toxicant exposure. High performance technologies including small molecule quantitative analysis, epigenomic, transcriptomic, and proteomic analyses are most frequently requested by CURES Center investigators and form the base for ESFC services. With Center investigators increasingly focused on questions regarding the interaction of environmental exposure and the physiological response to chemical and non-chemical stressors we have included analytical resources to quantify protein and DNA adducts, small molecule toxicants and metabolites, cellular profiling as well as cytokines, lipids and metabolites. Our combined facilities include some of the most advanced analytical capabilities available for targeted and untargeted analysis of the exposome and for discovery of exposure signatures. Although we focus on the common matrices of blood, plasma, urine, cells and tissues, we can work with any biological or environmental sample matrix. Because few investigators are familiar with the full scope and power of the technologies available through the ESFC we consult with investigators as they are planning a project. We offer advice regarding analytical end points, how those end points could be achieved experimentally and the cost involved in doing that work. To offset costs at the initial stages of a study 28 vouchers of $2500 are available each year. Vouchers can be used in any of the six ESFC labs. Investigators are rarely familiar with the latest advances in technology or which is most appropriate for the study. Omics technologies produce data sets that require specialized software available in the laboratory where the data are generated. Using our experience with bioinformatics for interpretation of the data is the final step offered by the ESFC. The complete ESFC package provides investigators with planning, advanced analytical technology, expert technical assistance and informatics.