Project summary Esophageal stratified squamous epithelia comprise proliferative basal cells that undergo terminal differentiation in the suprabasal cell layer. This homeostatic proliferation-differentiation gradient is regulated by Notch signaling that is impaired in eosinophilic esophagitis (EoE), a chronic inflammatory disorder characterized by mucosal eosinophilic infiltration, basal cell hyperplasia (BCH) and subepithelial fibrosis. BCH contributes to a barrier defect that facilitates lamina propria remodeling, culminating in fibrotic esophageal strictures that severely affect patients’ quality of life. While food allergen-activated immune cells and cytokines mediate EoE pathogenesis, the relationship between esophageal epithelial biology and EoE pathogenesis remains elusive. The ongoing project (R01-114436) has established the role of autophagy, a highly conserved cellular stress response, as a fundamental mucosal defense mechanism in EoE. Epithelial autophagy activation involves mitochondrial stress and reactive oxygen species (ROS) induced by proinflammatory EoE-relevant cytokines such as IL-13, which in turn stimulate BCH. BCH features depletion of CD73+ basal cells and reciprocal expansion of CD73- cells with low Notch activity, thereby limiting terminal differentiation. IL-13 promotes BCH by inhibiting Notch while inducing ROS-mediated mitochondrial (mt) damage marked by mtDNA depletion. Moreover, CD73- cells may maintain BCH via Notch-independent epithelial renewal. The overall objective in this competing renewal application is to elucidate the nature of basal cell fate regulation in EoE pathogenesis. The central hypothesis is that EoE-related inflammation influences basal cells’ activity to promote esophageal tissue remodeling. This hypothesis has been formulated based upon strong preliminary data and publications from the applicant’s laboratory and will be pursued through the following interrelated Specific Aims: (1) To elucidate how autophagy activation limits BCH in the EoE inflammatory milieu; (2) To delineate the role of mitochondrial damage in EoE-related BCH; (3) To unravel how Notch signaling protects against tissue remodeling in EoE. The present proposal utilizes a robust murine model of EoE with squamous epithelia- specific autophagy impairment, mtDNA depletion, or Notch inhibition with concurrent basal cell lineage tracing. These approaches are coupled with ex vivo esophageal 3D organoids from EoE mice and patient biopsies to define the mechanistic and functional role of autophagy and mitochondria in basal cell homeostasis. These innovative studies will reveal novel insights into esophageal basal cell biology and tissue remodeling. With genetically engineered mice, 3D organoids and patient biopsies, we will build a comprehensive platform for the development and validation of novel translational applications related to EoE therapy (autophagy activation and mitochondria-targeted antioxidants). These findings may have direct c...