# rPIV5- and AVLP-vectored vaccine development with public tumor-specific neoantigens

> **NIH NIH R01** · UNIVERSITY OF GEORGIA · 2023 · $146,672

## Abstract

Abstract
This application is being submitted in response to the Notice of Special Interest (NOSI) identified as NOT-CA-
23-045. The proposed supplement project aims to validate the immunogenicity of a total 10 public tumor-
specific neoantigen (pTSNA) candidates identified by our parent R01CA252713 project, entitled “Canine MHC-
I genotyping and tumor specific neoantigen determination”. These are defined as mutant peptides that are: 1)
derived from hotspot mutations in canine and human cancers; and 2) predicted to bind MHC class I (MHC-I)
alleles dominant in canine/human populations and/or subpopulations (breeds or ethnic groups).
We will collaborate with Drs. Biao He and Dong An, two leading vaccine developers to perform two studies. 1)
We will clone the pTSNA candidates in tandem into vaccine vectors parainfluenza virus 5 (PIV5) and PIV5-
based self-amplifying virus-like particle (AVLP). PIV5 and AVLP, developed by Drs He and An, are ideal
vaccine vectors especially for cancer vaccine development, as they elicit more robust cellular immune
responses, compared to other strategies. 2) We will infecting peripheral blood mononuclear cells (PBMCs)
isolated from dogs harboring the required MHC-I alleles with rPIV5-pTSNA and rAVLP-pTSNA viruses, identify
pTSNAs that are presented by the MHC-I alleles via mass-spectrometry, and identify immunogenic pTSNAs
via interferon-g assays.
This proposed supplement study will significantly enhance Aim 2c of the parent R01, by accelerating
immunogenic pTSNA discovery. Furthermore, the study will yield critical reagents (rPIV5-pTSNA amd rAVLP-
pTSNA vaccines) and knowledge for future cancer vaccine development that impacts a large population of
both dogs and humans. The study is especially significant, considering that PIV5-based vaccines are shown to
be safe and immunogenic for dogs, and PIV5-based vaccines are often administrated intranasally.

## Key facts

- **NIH application ID:** 10831315
- **Project number:** 3R01CA252713-03S1
- **Recipient organization:** UNIVERSITY OF GEORGIA
- **Principal Investigator:** William Hildebrand
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2023
- **Award amount:** $146,672
- **Award type:** 3
- **Project period:** 2021-05-11 → 2026-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10831315

## Citation

> US National Institutes of Health, RePORTER application 10831315, rPIV5- and AVLP-vectored vaccine development with public tumor-specific neoantigens (3R01CA252713-03S1). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10831315. Licensed CC0.

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