# Human Airway Epithelial Cell Culture Core

> **NIH NIH U19** · UNIVERSITY OF WASHINGTON · 2024 · $514,964

## Abstract

Core B: Airway Epithelial Cell Culture Core
Summary
The primary goal of Core B Airway Epithelial Cell Culture Core is to support the Seattle Center projects by
providing organotypic cultures of epithelial cells from carefully phenotyped individuals. The core is divided into
a pediatric sub-core and an adult sub-core. Core personnel will identify individuals scheduled for elective
surgery, explain the study, and obtain informed consent. Epithelial cells are collected from enrolled individuals
at the time of elective surgery by bronchial brushing via a secure endotracheal tube. Epithelial cells are
expanded in submerged culture and either stored or used immediately as needed for differentiated air-liquid
interface organotypic cultures to support the co-culture model systems in the two projects. A key function of the
Core is to carefully characterize individuals with asthma into T2-high and non-T2 clinical phenotypes, using
pre-defined criteria for children and adults. Both sub-cores will also collect epithelial cells from healthy controls
for comparisons. The pediatric sub-core will obtain additional longitudinal clinical data, allowing correlation of
specific clinical phenotypes and the associated epithelial endotypes with temporal clinical data. The adult sub-
core will also obtain airway cells via induced sputum, to assist in airway cell endotyping via determination of
the Type 2 Gene Mean (T2GM) and to provide airway cells for single cell RNA for sequencing. The adult sub-
core will also continue to expand a biorepository of adult airway epithelial cells obtained from donor lung
bronchial rings, which are discarded at the time of lung transplant. This provides significantly larger numbers of
cells per donor as compared to airway brushing. These cells will be provided along with assistance from the
core for mechanistic studies, using gene disruption techniques such as shRNA gene silencing or
CRISPR/Cas9 gene editing.

## Key facts

- **NIH application ID:** 10832552
- **Project number:** 5U19AI175089-02
- **Recipient organization:** UNIVERSITY OF WASHINGTON
- **Principal Investigator:** JASON S DEBLEY
- **Activity code:** U19 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $514,964
- **Award type:** 5
- **Project period:** 2023-04-25 → 2028-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10832552

## Citation

> US National Institutes of Health, RePORTER application 10832552, Human Airway Epithelial Cell Culture Core (5U19AI175089-02). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/10832552. Licensed CC0.

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