# Atherosclerosis core

> **NIH NIH P01** · NEW YORK UNIVERSITY D/B/A NYU LONG ISLAND SCHOOL OF MEDICINE · 2024 · $193,969

## Abstract

The Atherosclerosis Core (Core 3; C3) will be led by Dr. Edward Fisher and provide Program Project Grant
(PPG) investigators with three complementary approaches for analyzing atherosclerotic lesions, lesion
macrophages, and gene expression in myeloid cells and tissues investigated in the projects. First, C3 will
provide histological staining, immunohistochemistry, and quantification of atherosclerotic lesions. Second, it will
provide laser capture microdissection of macrophages and other types of cells in atherosclerotic lesions for
analysis of gene expression changes. Third, C3 will provide expertise in aortic digestion to obtain specific cell
compartments to provide samples to the NYU Genomic Technology Center (GTC) for RNA sequencing (RNA-
seq) and single cell RNA-seq (scRNA-seq) analyses. These `omic' data will then undergo bioinformatic
analyses under Core 1, which will oversee Administration, Data Management, Biostatistics, and
Bioinformatics.By providing a central laboratory, C3 offers these services with optimal efficiency and cost-
effectiveness, avoiding the need for PPG investigators to maintain the required instrumentation in their own
laboratories or use expensive commercial services. By centralizing and standardizing procedures, C3 provides
a common set of analytical tools to enable a unified understanding of molecular mechanisms involved in
pathophysiologic processes of atherosclerosis and the biology of macrophages and endothelial cells. The
Specific Aims of C3 are: 1. To provide comprehensive tissue analysis and quantification of atherosclerotic
lesions; 2. To provide laser capture microdissection (LCM) of target cells, primarily macrophages, in
atherosclerotic lesions for gene expression studies; 3. To prepare aortic tissue (by digestion and FACS) to
provide samples to the GTC for RNA-seq and scRNA-seq (with the resulting data to be analyzed by Core 1).

## Key facts

- **NIH application ID:** 10834204
- **Project number:** 5P01HL160470-02
- **Recipient organization:** NEW YORK UNIVERSITY D/B/A NYU LONG ISLAND SCHOOL OF MEDICINE
- **Principal Investigator:** Edward A Fisher
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $193,969
- **Award type:** 5
- **Project period:** 2023-05-01 → 2028-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10834204

## Citation

> US National Institutes of Health, RePORTER application 10834204, Atherosclerosis core (5P01HL160470-02). Retrieved via AI Analytics 2026-05-27 from https://api.ai-analytics.org/grant/nih/10834204. Licensed CC0.

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