# 100 Additional fresh samples for verification of assay to replace the original proposed banked samples that cannot be used due to repeated exposure to freezing and thawing.

> **NIH NIH SB1** · ACETAMINOPHEN TOXICITY DIAGNOSTICS, LLC · 2023 · $253,798

## Abstract

James, Laura P
Project Summary/Abstract
The original plan for development of AcetaSTAT was to use banked samples of serum collected
from acetaminophen overdose patients to verify the effectiveness of the assay prior to verification
and validation efforts. However, during the development of the work it has become apparent that
banked samples cannot be used due to their instability and repeated exposure to freezing and re-
thawing of samples. The assay is intended for immediate use by the clinician, so although it
appears to be sensitive to sample age, this will not impact the final intended use. However, the
lack of more recent samples has delayed the project and we have determined it will be necessary
to collect fresh samples from patients with acetaminophen exposure.

## Key facts

- **NIH application ID:** 10837949
- **Project number:** 3SB1DK079387-10S1
- **Recipient organization:** ACETAMINOPHEN TOXICITY DIAGNOSTICS, LLC
- **Principal Investigator:** Laura P James
- **Activity code:** SB1 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2023
- **Award amount:** $253,798
- **Award type:** 3
- **Project period:** 2008-04-01 → 2025-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10837949

## Citation

> US National Institutes of Health, RePORTER application 10837949, 100 Additional fresh samples for verification of assay to replace the original proposed banked samples that cannot be used due to repeated exposure to freezing and thawing. (3SB1DK079387-10S1). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10837949. Licensed CC0.

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