# Deciphering the Roles of Endometrial Glycogen Reserves and the Impact of Obesity on Fertility

> **NIH NIH R01** · UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN · 2024 · $391,864

## Abstract

Project Summary
Over 40% of pregnancies fail in women, most before or during embryo implantation. During this time, embryos
are dependent on secretions into the uterine lumen that contains all of the growth factors and nutrients
necessary for embryo development. In particular, embryos need glucose. From fertilization until the morula
stage, glucose uptake is low, but without glucose, the embryo degenerates. As embryos approach the
blastocyst stage, glucose uptake increases dramatically, providing ATP to the embryo within the hypoxic
uterine lumen. The endometrial stroma also uses a large amount of glucose to decidualize. After
decidualization, the stroma relies on Warburg metabolism to produce ATP and supplies glucose to the invading
embryo. Thus, it is clear that glucose availability must be regulated in a spatiotemporal manner to ensure a
successful pregnancy. Finally, obesity leads to reduced fertility in women through, in part, effects on the uterus
and its ability to support pregnancy. Most research on endometrial glucose has focused on the role of glucose
transporters. However, the endometrium can also transiently store glucose as the macromolecule glycogen. In
women, endometrial glycogen concentrations are correlated with fertility, but a direct link between uterine
glycogen and fertility has never been established. To investigate this in mice, we collected uteri on proestrus
and days post coitum (DPC) 1.5, 3.5, and 5.5. Our preliminary data show that the mouse endometrium stores
two distinct glycogen pools: in the uterine epithelium and the uterine decidua. Epithelial glycogen peaked at
proestrus and significantly reduced during the preimplantation period. The epithelial expressed glycogen
phosphorylase and glucose-6-phosphatase, suggesting the epithelium can catabolize glycogen and secrete
the resulting glucose. In contrast, the endometrial stroma stored little glycogen until decidualization, when
glycogen content increased 7-fold. Similarly, artificially induced decidualization in hormonally primed mice
resulted in a 5-fold increase in glycogen levels. In order to establish a definitive relationship between these
distinct pools of glycogen and fertility, we obtained glycogen synthase 1 (GYS1) floxed mice so that we can
knockout GYS1 in a tissue-specific manner. Aim 1 will generate an LTFiCre/+ GYS1flox/flox mouse to study the role
of epithelial glycogen in glucose secretion and preimplantation embryo survival. Aim 2 will use AMHR2Cre/+
GYS1flox/flox mice to study the role of glycogen in the decidua. Collectively these aims will establish a causative
link between the ability of the uterus to store glycogen in the epithelium and decidua and fertility. Aim 3 will use
a diet-induced obesity model to study how maternal obesity affects glycogen metabolism in the mouse uterus.
We will determine how a 45% and 60% fat diet affects uterine metabolism and fertility in a C57BL/6 mouse
model. In vitro, we will determine the effects of hyperglycemia ...

## Key facts

- **NIH application ID:** 10838487
- **Project number:** 5R01HD111706-02
- **Recipient organization:** UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
- **Principal Investigator:** Matthew J Dean
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $391,864
- **Award type:** 5
- **Project period:** 2023-05-08 → 2028-02-29

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10838487

## Citation

> US National Institutes of Health, RePORTER application 10838487, Deciphering the Roles of Endometrial Glycogen Reserves and the Impact of Obesity on Fertility (5R01HD111706-02). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10838487. Licensed CC0.

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