# Cell migration and wound repair

> **NIH NIH R35** · UNIVERSITY OF WISCONSIN-MADISON · 2024 · $669,792

## Abstract

ABSTRACT
The overall goal of this Maximizing Investigator’s Research award renewal application is to understand the
integration of complex signaling networks at both the single cell and multi-cellular level during wound healing.
Despite progress in understanding the signals that guide wound repair, there remains a significant gap in
understanding how different types of cells communicate to integrate a wound healing response. This gap limits
our ability to design new therapeutic strategies for a broad range of human disease including diabetes, cancer,
cardiovascular disease and autoimmunity. The overall focus of our research is to understand the basic
molecular mechanisms that regulate cell migration and how defects in cell migration contribute to
human disease in the context of tissue damage and repair. The optical transparency and ease of genetic
manipulation make zebrafish an ideal model system to dissect multi-cellular and tissue interactions during wound
repair. During the prior funding period, we invested significant effort in developing new tools for imaging and
manipulating cell dynamics, cell guidance cues, metabolism and matrix remodeling during wound healing in both
simple and complex wound models. We performed large-scale TRAP (translating ribosomal affinity purification)-
RNAseq and identified context- and cell-type specific gene expression changes. Using genome editing we are
now poised to uncover new signaling mechanisms and guidance cues that regulate neutrophil reverse migration
and inflammation resolution, and influence matrix remodeling during wound healing. Understanding how
wound repair is orchestrated and integrated at both the single cell and multi-cellular level, including the
role of microbial interactions, in the different types of tissue damage is the focus of our future research.
The overall goal of our work is to identify key signaling networks and guidance cues that mediate cell migration
during wound repair, dissect how they are altered in pathological conditions and ultimately may be targeted to
understand and treat human disease.

## Key facts

- **NIH application ID:** 10838534
- **Project number:** 5R35GM118027-09
- **Recipient organization:** UNIVERSITY OF WISCONSIN-MADISON
- **Principal Investigator:** Anna Huttenlocher
- **Activity code:** R35 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $669,792
- **Award type:** 5
- **Project period:** 2016-06-01 → 2026-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10838534

## Citation

> US National Institutes of Health, RePORTER application 10838534, Cell migration and wound repair (5R35GM118027-09). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10838534. Licensed CC0.

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