# Analytical and Biophysical Research Core

> **NIH NIH P20** · UNIVERSITY OF MISSISSIPPI · 2024 · $364,632

## Abstract

Project Summary/Abstract
The University of Mississippi proposes to establish the Glycoscience Center of Research Excellence
(GlyCORE), a Phase I Center of Biomedical Research Excellence. Glycoscience is an essential and rapidly
emerging field of biomedical science, and the challenging nature of glycoscience research requires tools and
expertise that are not commonly found in the biomedical research community. Moreover, the biomedical
community is rapidly growing to appreciate the essential role of carbohydrates, with researchers outside of the
self-identified “glycoscience” community finding themselves faced with addressing challenging questions in
glycoscience as part of the course of their biomedical researchers. The focus of the Analytical and Biophysical
Research Core is three-fold: to train investigators new to glycoscience in proper carbohydrate sample
preparation and handling; to support hypothesis-driven research with targeted quantitative analyses from
both enriched samples and complex mixtures; and to support discovery-based research to give investigators
a system-level view of changes upon perturbation of targeted systems. Training of researchers is a key
function of the Core, especially in Phase I when the institutional level of expertise in glycoscience is in its
earliest stage of development. Core 1 will train researchers in carbohydrate and carbohydrate conjugate
sample preparation techniques. Targeted analyses will be provided as a key technology for hypothesis-driven
research, where an investigator wants to test for the presence and quantity of a given glycan or glycoside,
structurally characterize an unknown molecule, or validate a key biological or chemical resource. Structural
analyses of glycan modification, protein-carbohydrate interactions, binding affinity estimations, and other
biophysical analyses will be supported through MS-, LC- and spectrophotometry-based technologies. Access
to NMR instrumentation and expertise in spectral acquisition and interpretation will also be provided. Finally,
Core 1 will offer “omics”-style discovery-based surveys of glycomes, proteomes, metabolomes, etc. in
quantitative, semi-quantitative, and qualitative fashions, supporting systems glycobiology and hypothesis
development for targeted assay design. Core 1 will interface closely with the Computational Chemistry and
Bioinformatics Core to provide advanced statistical and pathway analyses for “omics” data. The services of
Core 1 will use both existing University of Mississippi resources as well as a dedicated high resolution Thermo
Q-Exactive Plus LC-MS/MS system and a Dionex Ultimate 3000 analytical UHPLC system. Due to the poor
UV-absorption characteristics of most carbohydrates, fluorescence detection for tagged carbohydrates and
charged aerosol detection of underivatized carbohydrates will also be available for sensitive detection and
quantitation at high dynamic range. By focusing our Phase I Analytical and Biophysical Core on a small
n...

## Key facts

- **NIH application ID:** 10838583
- **Project number:** 5P20GM130460-05
- **Recipient organization:** UNIVERSITY OF MISSISSIPPI
- **Principal Investigator:** Joshua S Sharp
- **Activity code:** P20 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $364,632
- **Award type:** 5
- **Project period:** 2020-05-15 → 2025-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10838583

## Citation

> US National Institutes of Health, RePORTER application 10838583, Analytical and Biophysical Research Core (5P20GM130460-05). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10838583. Licensed CC0.

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