# Aryl hydrocarbon receptor and bilirubin as therapeutic target for ICH

> **NIH NIH R01** · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · 2024 · $457,184

## Abstract

After intracerebral hemorrhage (ICH), phago(endo)cytosis of hematoma components [erythrocytes (RBC),
hemoglobin (Hb) and heme], by microglia/macrophages (MMΦ) is essential for removal/detoxification of the
hematoma, resolution of inflammation, and restoration of brain homeostasis for optimal neurological recovery.
Thus, strategies for protecting MMΦ from injury during engulfment of toxic hematoma components is critical for
the efficiency of MMΦ in clearing the hematoma after ICH.
Upon engulfment of RBC/Hb/heme from the hematoma, MMΦ express heme oxygenase 1 (HO1; encoded by
Hmox1), which catabolizes heme to produce large amounts of bilirubin (BrB) within MMΦ. Normally BrB is a
beneficial and potent antioxidant; however, when intracellular BrB accumulates to very high concentrations, it
may reversibly inhibit HO1 activity, thus reducing MMΦ' function and even precipitate onto biological membranes
or nucleic acids (due to its poor water solubility), causing injury and compromising MMΦ' integrity.
On the cellular level, BrB acts as an endogenous agonist for a pleotropic transcription factor, aryl hydrocarbon
receptor (AhR), a protein that, based on prior research and our preliminary data, plays essential roles in
supporting MMΦ for their integrity and function during hematoma clearance to enhance brain recovery after ICH.
Our preliminary studies established that intracellularly elevated free BrB in microglia (MΦ), during
erythrophagocytosis, activates AhR to promote transcription of BrB handling proteins, ligandins
[(LGN/glutathione S-transferases), which bind fBrB to increase its cytosol solubility] and multidrug resistance
protein 1 [(Mrp1)14, 15, which mediates efflux of BrB from the intracellular compartment to the extracellular space].
Without fast clearance of intracellularly accumulating fBrB, MΦ are injured and function less efficiently, and
produce large amount of proinflammatory factors that could cause severe brain damage. In addition, we
established that BrB-activated AhR promotes phagocytosis and upregulates the expression of nuclear factor
erythroid 2-related factor 2 (Nrf2), a master regulator of cellular oxidative defense for hematoma detoxification,
and a potential therapeutic target for ICH. Furthermore, we established that AhR activity regulates RelB nuclear
translocation, suggesting that AhR may also interact with RelB to transactivate genes involving inflammation
resolution. Ultimately, using the mouse model of ICH, we found very promising therapeutic benefits of AhR
agonist ITE19-21, alone and even more so in combination with the activator of Nrf2 (sulforaphane, SF), regarding
neurological outcome and hematoma clearance.
Our hypothesis is that after ICH, activation of AhR/RelB in MMΦ during hematoma removal by BrB (or other
non-toxic AhR activators) is vital for preserving MMΦ' survival/phagocytic functions, avoiding self-inflicted
damage, retention of reparative phenotype, ultimately leading to a more efficient MMΦ-mediated he...

## Key facts

- **NIH application ID:** 10839435
- **Project number:** 5R01NS119804-04
- **Recipient organization:** UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON
- **Principal Investigator:** Jaroslaw Aronowski
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $457,184
- **Award type:** 5
- **Project period:** 2021-06-01 → 2026-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10839435

## Citation

> US National Institutes of Health, RePORTER application 10839435, Aryl hydrocarbon receptor and bilirubin as therapeutic target for ICH (5R01NS119804-04). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/10839435. Licensed CC0.

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