# Component for Institution # 264313

> **NIH NIH P30** · TEMPLE UNIV OF THE COMMONWEALTH · 2024 · $188,737

## Abstract

The Molecular Biology Core is a critical component of the NIDA P30 Center at Temple
University. The Molecular Core offers services to investigators that involve either short- or long-
term use of molecular techniques. This Core provides three types of services. First, the Core
supports the design and construction of molecular constructs for the expression of genes of
interest. This will include, for example, the expression either in vitro or in vivo of opioid or
cannabinoid receptors. These molecular constructs will permit a variety of studies of interest to
collaborating investigators including the analysis of receptor-mediated signaling processes and
the analysis of the cellular trafficking of receptors (or other genes of interest). Second, the
Molecular Biology Core will provide gene expression analysis both in vitro and in vivo. The Core
provides highly quantitative analysis of mRNA and miRNA expression using RT-PCR, northern
blot analysis, RNase protection analysis, and new to this renewal application, the novel FISH
flow (PrimeFlow) analysis. The Core also offers comprehensive transcription factor function
analyses. Third, the Core provides genotyping services for the Animal Core and to other
investigators breeding mutant animals. Services are provided for investigators who span a
variety of disciplines and work is carried out in direct collaboration with the investigator. The
Molecular Biology Core is particularly interested in continuing efforts to promote the use of
molecular techniques to study the role of drugs of abuse in the host-parasite interaction with
HIV. The Core has been highly successful in engaging NIH-funded investigators from many
disciplines in collaborative research projects that have advanced the understanding of the
actions of drugs of abuse and their interplay with the immune system and with HIV. By
combining the expertise of investigators with diverse interests, new perspectives and potentially
novel investigations can be developed.

## Key facts

- **NIH application ID:** 10839858
- **Project number:** 5P30DA013429-25
- **Recipient organization:** TEMPLE UNIV OF THE COMMONWEALTH
- **Principal Investigator:** Thomas J Rogers
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $188,737
- **Award type:** 5
- **Project period:** 2000-09-30 → 2025-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10839858

## Citation

> US National Institutes of Health, RePORTER application 10839858, Component for Institution # 264313 (5P30DA013429-25). Retrieved via AI Analytics 2026-05-21 from https://api.ai-analytics.org/grant/nih/10839858. Licensed CC0.

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