The overall objective of the CRISPR, Vector and Transgenic Mouse Core (CVTMC) is to provide Diabetes Research Center (DRC) affiliate investigators at the University of Washington (UW) and the Greater Seattle area with state-of-the-art vectors necessary to edit, monitor, or alter expression of genes and RNAs of interest in cultured cells, tissues, and animals. This well-used Core has evolved considerably since the last competitive renewal, based on the changing needs of affiliate investigators. New and more efficient CRISPR methods have been developed, use of viral vectors containing cell type-selective promoters has been greatly expanded, and more sophisticated virus production and purification methods have been implemented. These newer services are highly used, and now provide the bulk of the Core’s work. Conversely, services that are less cutting-edge and are no longer frequently requested have been removed. The specific aims of the Core are to provide the following services to affiliate investigators: (1) Production of CRISPR reagents for genome editing and for various other applications, such as CRISPR-mediated transcriptional activation and repression; (2) Facilitate generation of mouse models by CRISPR through collaboration with the Transgenic Resources Program at the UW; (3) Facilitate generation of cell lines by CRISPR in collaboration with the Institute for Stem Cell and Regenerative Medicine (ISCRM) at the UW; (4) Production of adeno-associated viral (AAV) and lentiviral vectors for use in animals, tissues and cells; (5) Development of genotyping methods to validate edited mice and cell lines; and other specialized molecular biology, such as cloning and PCR; and (6) Consultation at each step and training of affiliates and their trainees in the Core’s methods. The Core has been highly productive in the current funding period and has added new services that are expected to significantly increase productivity and usability for DRC affiliate investigators to meet the Center's goal to enhance research in diabetes, obesity and related disorders in the Greater Seattle area and beyond.