ABSTRACT - OVERALL The University of Washington (UW) Center for Translational Muscle Research (CTMR) will continue to provide critical resources and programs to enhance productivity of investigators to address multi-scale and multidisciplinary research of muscle disease and treatment strategies. Our research and clinical faculty at the UW, Seattle Childrens and the Fred Hutch Cancer Research Center have expertise in muscle disease and aging, exercise, and neuromuscular plasticity. The overall goal of the CTMR is to provide a unifying force to this Seattle area research community and beyond that accelerates innovative research by providing research tools, facilities, and expertise in a combination available only at the UW. This will facilitate novel insights into muscle pathologies and move new therapeutics towards the clinic and the marketplace. The CTMR offers 4 cores, one administrative and three research resource cores. The Administrative Core (A) provides program management and enrichment through symposia and workshops, seminar series, training and educational opportunities for new investigators and more experienced investigators moving into muscle research. It also administers a very successful Pilot Awards program that provides funding resources to test ideas, develop approaches and collect data that can be used for follow-on extramural research applications or ongoing projects. The Resource Cores feature multi-scale (physical and temporal) measurement and analytical tools. The Mechanics and Devices Core (B) provides state of the art measurements of muscle biomechanics at multiple levels of integration and develops new assays for maturation and assessment of early-stage muscle. The Metabolism and Energetics Core (C) provides tools for in depth measures and analysis of metabolomics, energetics, cell respiration and mitochondrial function. The Quantitative Analysis Core (D) provides computational and statistical tools for understanding mechanisms of function and dysfunction with disease and suggesting new therapeutic targets. The ability to gain information at multiple scales - from protein to organelles, cells, tissue, and organ - across energetic, metabolic, structure and functional domains using experimental and quantitative analytic tools with integrative capacity is a powerful approach that is not easily available to an individual investigator or research group. Such an interdisciplinary approach offers the capacity to accelerate research to better understand skeletal muscle aging, injury and diseases and speed therapeutics development. In the first 4 years, the CTMR has been significantly impactful on training and establishing new investigators in muscle research and facilitating movement of more established investigators into new research areas. The Resource Cores have developed or purchased new instrumentation and assays to meet the changing needs of CTMR faculty. In this renewal proposal we summarize forward looking efforts to rem...