# A Functional Analysis of Arginine Methylation

> **NIH NIH R35** · UNIVERSITY OF TX MD ANDERSON CAN CTR · 2024 · $405,000

## Abstract

PROJECT SUMMARY/ABSTRACT
Our goal is to broadly understand the biological roles of arginine methylation, a very common post-translational
modification (PTM). This PTM is deposited by at least nine protein arginine methyltransferases (PRMTs) in
mammals. Like lysine methylation, arginine methylation of a substrate often recruits an effector molecule to the
newly created methyl-motifs. Unlike lysine methylation, relatively few effects have been identified for
methylarginine marks. This paucity of identified methylarginine (Rme) mark effectors and the signaling roles of
the known effectors define a knowledge gap that will be addressed here. By performing proteomic screens, we
have identified a novel reader for Rme marks, called SART3. This effector does not harbor a Tudor domain
which is usually found in “traditional” readers of Rme marks, but rather it carries a series of HAT (Half-a-TPR)
repeats that are rich in aromatic amino acids. Here, we plan to characterize this interaction and investigate the
possibility that other TPR repeat-containing proteins may be involved in sensing arginine methylation. Also, we
are investigating the functions of two known effects of Rme marks, called SND1 and TDRD3. Through a
combination of mouse work and protein array studies, we have determined that SND1 can likely directly activate
the kinase activity of S6K2, and that TDRD3 can play a role in the DNA damage response.

## Key facts

- **NIH application ID:** 10842017
- **Project number:** 1R35GM153387-01
- **Recipient organization:** UNIVERSITY OF TX MD ANDERSON CAN CTR
- **Principal Investigator:** MARK T. BEDFORD
- **Activity code:** R35 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $405,000
- **Award type:** 1
- **Project period:** 2024-09-01 → 2029-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10842017

## Citation

> US National Institutes of Health, RePORTER application 10842017, A Functional Analysis of Arginine Methylation (1R35GM153387-01). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10842017. Licensed CC0.

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