# Interrogating Complex Methylome Network with Integrated Chemical Biology Tools

> **NIH NIH R35** · SLOAN-KETTERING INST CAN RESEARCH · 2024 · $799,040

## Abstract

Project Summary/Abstract: Epigenetic biology associates with inherited cellular cues to define
diverse cell fates with the same genome. Among essential epigenetic events is protein methylation
deposited by more than 60 human protein methyltransferases (PMTs) and recognized by hundreds
of effectors to render downstream functions. Because many methylation events are invisible in
cellular contexts for conventional methods, our understanding of epigenetic roles of protein
methylation is very limited. In the past five years, our laboratory has focused on developing novel
chemical biology technologies and implementing them to annotate novel methylation events and
their downstream outcomes. We plan to continue this research theme for technological, functional,
and conceptual advances. Regarding the technological advance, we envision developing a
collection of complementary chemical biology tools to annotate each PMT-target-effector axis in
the context of the complex methylome. For potential functional and conceptual advances as
supported by our preliminary data, we will characterize nonhistone and noncanonical histone
methylation events associated with transcriptional regulation and protein homeostasis. The
completion of this proposal is expected to reveal the molecular mechanisms of multiple protein
methylation events in their biologically relevant contexts.

## Key facts

- **NIH application ID:** 10842880
- **Project number:** 2R35GM131858-06
- **Recipient organization:** SLOAN-KETTERING INST CAN RESEARCH
- **Principal Investigator:** Minkui Luo
- **Activity code:** R35 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $799,040
- **Award type:** 2
- **Project period:** 2019-06-01 → 2029-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10842880

## Citation

> US National Institutes of Health, RePORTER application 10842880, Interrogating Complex Methylome Network with Integrated Chemical Biology Tools (2R35GM131858-06). Retrieved via AI Analytics 2026-06-11 from https://api.ai-analytics.org/grant/nih/10842880. Licensed CC0.

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